| Object:Study the effection of periodontitis on erectile function in rats. Methods:1.10 healthy male 4-week-old Sprage-Dawley rats were randomly divided into 2 groups:periodontitis group (A group), control group (B group).The periodontitis group was ligatured bilateral first and second maxillary molars by 20 steel wires. Imbed silk thread which was steeped by 1mg/ml LPS into periodontal pocket.2.At 8 weeks, all rats detection periodontal status, underwent erectile function testing by measuring maximum intracavernous pressure/mean arterial pressure (ICPmax/MAP), collect blood,maxillary distal alveolar bone and corpora cavernosa penis.3.The left side maxillary distal alveolar bone were Stained by HE,the inflammation was measured with histological activity index. The right side maxillary distal alveolar bone was removed gum,To measure of bone height around the roots.4.Serum TNF-αand CRP were detected, NOS activity and cGMP content were examined, eNOS was analyzed by western blot, eNOS mRNA were detected by real-time PCR.5. Statistical methods:The results were showed by means plus or subtracting stand deviation. Means between the Model groups and Control groups were compared by Independent-Samples T test. All the statistical methods were accomplished by software SPSS 17.0. Result:1.A group rats represented obvious and typical periodontitis in clinical and histopathological aspects.2.The weight and blood sugar in group A[(322.2±24.88)g,(5.38±0.54)mmol/L] were no significantly defference with group B[(335.8±19.58)g,(5.68±0.94) mmol/L] (P=0.365,P=0.552). Haemoglobin and red blood cell in group A[(160.00±9.92) g/L,(8.71±0.90) 10E12/L] were no significantly defference with group B[(166.00±3.40)g/L,(8.97±0.19)10E12/L] (P=0.248,P=0.564). while blood cell in group A[(13.00±0.93)10E12/L] significantly increased with group B[(7.01±1.88) 10E12/L] (P＜0.001).3.The ICPmax/MAP×100 in group A was significantly less than in group B at 3 V (9.54±6.16,and 30.45±3.12,respectively) and at 5V(30.91±5.61,and 50.52±9.52,respectively) stimulation voltage,respectively (P=0.018,P<0.001).4.The serum TNF-αand CRP in group A[(433.10±69.33) pg/ml,(52.67±26.04)ng/ml] was significantly increased than in group B[(208.97±69.23) pg/ml, (22.55±16.36) ng/ml](P<0.001,P=0.006).5.The NOS activity and cGMP content in group A[(1.02±0.48)U/mgprot, (35.86±11.73) pmol/mg] was signifycantly less than in group B[(2.95±1.43)U/ mgprot, (50.50±12.17) pmol/mg](P=0.037, P=0.016).6.The expression of eNOS mRNA in group A[3.47±1.95] was no significantly defference with group B[2.54±0.67] (P=0.339).7.The expression of eNOS in group A[0.58±0.07] was significantly less than in group B[0.70±0.08](P=0.048). Conclusion:Periodontitis may mediated erectile dysfunction though the expression of eNOS in corpus cavernosum of rats, simultaneously decrease the activity of eNOS, NOS and the content of cGMP and NO. |
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