The Expression Of Chemokine IL-10, IL-12 And Chemokine Recpetor CCR5, CXCR4 Of Leprosy And Human Immunodeficiency Virus-1 Co-infected Subjects

Objective:To investigate the changes of immune status after leprosy and HIV infection in order to further improve the prevention and treatments of leprosy and AIDS on a scientific basis. Methods:3 cases of leprosy and HIV co-infection,9 cases of HIV infection,10 cases of leprosy infection and 9 cases of healthy controls, then we used flow cytometry to detect the expression of chemokine receptor CCR5 and CXCR4 on the surface of CD4+and CD8+T cells, and its relationship with the relevance of viral load; We take the method of double antibody sandwich ELISA for the detection of serum cytokine IL-10, IL-12 levels; at last we used real time quantitative PCR (RT-PCR) to check HIV viral load. Results:(1) The CD4+T cell counts and CD4/CD8 ratios were significantly decreased (p<0.05) in the group of leprosy and HIV co-infection, and the group of HIV infection. The ratio of CD4/CD8 in HIV infection group was lower compared with the group of leprosy and HIV co-infection (p<0.05). Further stratification analysis of HIV infection did not show statistically significance (p>0.05). (2)The expression of CCR5 on CD4+T cells surface increased slightly in the group of leprosy and HIV co-infection and the group of HIV infection, while the expression of CCR5 on CD8+T cells surface decreased slightly and did not show statistically significance (p> 0.05), There was no correlation between viral load and the expression of CCR5 on CD4+T cells surface(r=0.119, p>0.05). (3)The expression chemokine receptor CXCR4 on the surface of CD4+ T cells was not significantly different in the group of Leprosy and HIV co-infection, leprosy infection, HIV infection and healthy control (p> 0.05). (4)The expression of IL-10 were increased in the group of leprosy and HIV co-infection, HIV co-infection, and leprosy infection, but there was no statistically difference compared with the healthy group (p> 0.05). The levels of IL-10 in serum was positively correlated with the expression of CCR5 on CD4+ T cells surface in the group of HIV infection (r=0.762 p=0.028). (5) The IL-12 levels in serum were reduced in the leprosy and HIV co-infection group and HIV infection group, but did not show statistically significance (p>0.05). Conclusion:(1) The immune function of HIV mono-infection and leprosy and HIV co-infection is decreased. Compared with HIV mono-infection group,leprosy and HIV co-infection group maybe not show serious dysfunction of immune system.(2)There are high expression of CCR5 and low expression of CXCR4 on CD4+T cells of leprosy and HIV co-infection, which indicated that infection of HIV maybe an important factor to influence on the expression of CCR5 and CXCR4 on CD4+T cell. (3)The level of IL-10 is increased slightly and the level of IL-12 is decreased.This results was no significant compared with healthy group control,but this still firstly illustrated that the function of differentiation of ThO to Thl is reduced,and there are a current of Th1/Th2 balance to Th2. (4)The expression of CCR5 on CD4+T cells was correlated positively with the level of IL-10 in HIV infection.(5)the problems of this experiment is that the simple number is small about HIV/leprosy coinfection group.So we can take more study about the results by scale up simple.