The Expressions And Significance Of Prox-1 And PCNA In Glioma

Objective: To explore the expression of prox-1 in human glioma cell of patients with neurogliocytoma and to see whether or not prox-1's expression associate with the differentiation and pathologic grading. It was expected that this study could reveal some essential qualities of the glioma and provide potential application for judging the malignant grades and biological treatment; And to investigate the expressions and significance of prox-1 and PCNA in glioma, and to analyze the relationship between the expressions and malignant degree.Methods: Prox-1 was detected by western-blot in 65 cases of human glioma and 13 specimens of normal brain tissues. Immunohistochemical S-P method was employed to examine the expression of Prox-1 and PCNA in 65 cases of glioma, 13 cases of normal cerebral tissue as control group. Meanwhile the relationship of Prox-1 and PCNA expression was analyzed.Results: (1)There was a significantly positive correlation between the expression of Prox-1 and the grade of glima. (2) The expression rates of Prox-1 and PCNA in human brain glioma tissues were significantly higher than those in normal brain tissues. The protein expression of Prox-1 and PCNA increased with the increasing of pathological grade of glioma, there were significantly difference between the groups(P<0.05).(3) There was a significantly positive correlation between the expression of Prox-1 and PCNA protein(r=0.758,P<0.001).Conclusion: Prox-1 is overexpressed in malignant gliomas ,especially in glioblastoma tissue , which means that it may relate to the progression and invasion of the tumor , and may be regarded a useful marker for judging the malignant phenotype of glioma.