P53 And Its Downstream Gene METTL-9 Expression In Colon Cancer

Background: Colon cancer development is a multi-step, multi-stage and multi-genes involved in genetic disease. P53 tumor suppressor gene inactivation in colon cancer, advanced adenoma to play an important role in the transformation process. METTL-9 is another newly discovered downstream of P53 gene, the gene may be mainly through the activation of P53 protein with its specific sequence, thus promoting or inhibiting the gene transcription and translation. Through analysis by fluorescence quantitative PCR, in colon of P53 and METTL-9 gene expression and explore their relationship with the development of colon cancer.Objective: Explore the P53 gene and mettl -9 in the tissue of the colon cancer, cancer of the tissues and other tissues of the present conditions and study its the matter with the colon cancer. Methods: Look at the morphologic situation of histiocyte after HE stained; Apply the real-time fluorescent quantitation PCR to detect the gene expression of P53 and METTL-9 in the tissue. Results:The expression of P53 gene in control tissues was significantly higher than the cancer tissues'and the adjacent tissues'(p<0.05); the expression of P53 gene in cancer tissues was significantly lower than the adjacent tissues'; in control tissues, METTL-9 gene expression was significantly lower than the cancer tissues'and the adjacent tissues'(p<0.05); P53 gene expression in cancer tissues was significantly lower than the adjacent tissues('p<0.05); in cancer tissues, P53 gene and METTL-9 gene were negative.Conclusion: The mutations of P53 gene in the course of colon cancer has played an important role; its downstream gene METTL-9 is highly expressed in colon cancer, and the 9-methyl-converting enzyme which produced by Transcription and translation may lead gene hypermethylation in the colon cancer, and thereby promote the development of colon cancer.