| Obeictive: 1. By means of interventional treatment, investigating the feasibility and safety of microchemoembolism (MCE) in colon carcinoma. To observe the morphologic pharmacokinetic changes after MCE To provide the theoretic basis for improving the therapeutic effect of artery infusing chemotherapy and modifying MCE method on colon carcinoma. 2. Observing the reversion effect of multidrug resistance gene (MDR1) of cyclosporin A (C5A) in vitro and in vivo, to provide the theoretical basis for improving the chemotherapy effect of colon carcinoma, improving the chemotherapeutic agent remedy. PART L Feasibility and safety of microchemoembolus perfusion in the Inferior Mesenteric Artery. Materials and Methods: 20 mature hybrid dogs were divided into two groups: 1. In control group (10 dogs), medical aotivated carbon microspheres (CH) with the diameter of 30 ?60 ?m(group A), emulsified lipiodols with the diameter of 1 0?0 i m(group B), 5 dogs respectively. 2.In experiment group( 10 dogs), five were treated with microembolism(group C). The other five were treated with microchemoembolism M(CE) (group D). The emboli were CH with the diameter of 10?0 ?m, and the chemotherapeutic agents was FAM. All the dogs were catheterized in the inferior mesenteric arteries and injected with emboli. Post-operation effects were observed. Embolized and un- embolized segments of colon walls were taken as specimen. Liver and lymph nodes were observed under optic and transmission electron microscope. Autopsy were performed in the end. Result: In the control group, embolized colon segiments all perished. All the 10 dogs died within 12挆72 hours after operation, while in the experiment group, transient degeneration in the mucous and submucous layers (ML,SML) were observed. Small focal necrosis took place rarely. Exudation of inflammatory cells were found in the colon wall, most of which were in the SLM The changes mentioned above V appeared more in group D than in group C. Lesions recovered completely after two weeks, while the microemboli in the liver, lung, kidney and lymph nodes remained. Conclusion: Colons of the dogs can tolerate MCE of CHEL 10? 20 Pm. PART II. Effects of Perfusing Medical Activated Carbon Microspheres in the Inferior Mesenteric Artery. Materials and Methods: Six mature hybrid dogs, anesthesized, got inferior mesenteric arteries catheterized (with the Shelding抯 methods), and got transabdominal portai vein catheteriza-tion. Simple contrast medium and the contrast medium mixed with CHCP l0?0 ii m were detected under DSA. The concemtration of 5-Fu in the portal vein were detected with Liquid Chromatography and marked in scatter diagram. The half life times of 5-Fu after simple artery perfuion chemotherapy or after MCE were calculated and analyzed with t test (in Microsoft Excel). Dosage of 5-Fu was 20mg/kg, that of C11 suspension was 0.3mlIkg, that of contrast medium was 0.SmlIkg, the velocity of injecting 5-Fu with high pressure injector was 2m1/second. 3 hours after the MCE, embolized colon segment were taken and observed under optic and transmission electron microscope. Two weeks later, autopsy was performed. Result: The development time of simple contrast medium after injection was 6.25 ?1.50 seconds. The arteries looked smooth. The vessels spasmed and curvated shortly after perfusion... |
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