The Cell Morphology And The Expression Of TNF-α In Human Periodontal Ligament Infected By H.pylori

[Objective]: Establishing the model of Helicobacter pylori (H.pylori) infects human periodontal ligament fibroblasts (PDLFs) in vitro, observes the morphological of PDLFs and the secretion of TNF-αexpression and change, in order to observe the relationship between them from the way of the cytokine and provide laboratory evidence to H.pylori and periodontal disease .[Mthods]:1. Take advantage of PDLFs culture in vitro, the cell concentration adjusted to 2×10~5/ml;2. Take advantage of H.pylori culture in vitro, H.pylori were divided into 0 CFU / ml, 1×10~8CFU/ml, 2×10~7 CFU / ml, 4×10~6 CFU / ml, 8×10~5 CFU / ml five groups;3.Establishing the model of Helicobacter pylori (H.pylori) infects human periodontal ligament fibroblasts (PDLFs) in vitro, coculture 0-48h, using inverted microscope observed the morphological changes of PDLFs and detected the infected cell culture supernatant TNF-αexpression by ELISA.[Results]:1. With the increasing of H.pylori concentration and the role of time, PDLFs cell morphology from the spindle into a round adherent, adherent cells decreased, suspended cells increased and cell debris appeared around.2. PDLFs incubated with different concentrations of H.pylori after 6h, 12h, 24h, 36h, 48h compared with the control group, TNF-αvalues were higher (p <0.05). In the case of the same time, TNF-αexpression was increased first and then decreased after exposure to 8×10~5-1×10~8CFU / ml, and peaked at the concentration of 4×10~6CFU/ml. In the case of the same H.pylori concentration, TNF-αexpression was increased first and then decreased after exposure to 6-48h, and reached the best results in 12h. [Conclutions]: Helicobacter pylori in vitro can change the human periodontal ligament fibroblast morphology and induced the production of TNF-α. H.pylori associate with TNF-αinvolved in the pathogenesis of periodontal disease and played a very important role on periodontitis.