Explore Of Relationship Between HBV CccDNA,HBV TDNA Load In Hepatocyte And Hepatocellular Carcinoma Related HBV Infection

Objective: To explore relationship between HBV cccDNA,HBV tDNA load in hepatocyte and hepatocellular carcinoma related HBV infection, and provide theory for prevention and treatment of primary liver cell carcinoma.Methods: HBV cccDNA and HBV tDNA in biopsy liver tissue from 33 cases with chronic HBV infection (CHB infection group) and in the cacer tissue(cancer group) and cancer-adjacent tissues (cancer-adjacent group) from 60 cases with hepatocellular carcinoma (HCC) related HBV were detected using fluorescence quantitative polymerase chain reaction method ; HBsAg, HBcAg of liver tissue were measured by immunohistochemical. The inflammation activity and fibrosis degree were scored in tissue sections with HE dyeing. The results where analyzed by SPSS16.0 statistics software. The correlation between hepatocytes HBV cccDNA,HBV tDNA and sera HBV DNA in 3 group were anlysised respevtively; Compared with the load of hepatocytes HBV cccDNA,HBV tDNA,cccDNA/HBV tDNA among 3 groupsrespectively; Compared with the load of hepatocytes HBV cccDNA,HBV tDNA among 3 patterns of HBsAg,HBcAg expression; The correlation between hepatocytes HBV cccDNA,HBV tDNA and the degree of inflammation and fibrosis in CHB infection group and cancer-adjacent group were anlysised respevtively.Results: (1) There was no obvious correlation between hepatocytes HBV cccDNA,tDNA load and sera HBV DNA load in cancer and cancer-adjacent groups. ( r=0.165,P=0.206 , r=0.122,P=0.352 respectively); low degree positive correlation between hepatocytes HBV cccDNA,tDNA load and sera HBV DNA load in CHB infection group. (r = 0.356, P = 0.042, r = 0.496, P = 0.003 repectively); (2) hepatocytes HBV cccDNA, HBV tDNA in cancer tissue were higher than cancer-adjacent tissues(P=0.000,0.042),was similar to CHB infection group(P=0.4,0.446); hepatocyte HBV cccDNA, HBV tDNA in CHB infection group were significantly higher than cancer-adjacent tissues,(P=0.041,0.001 repectively); (3) The ration of cccDNA/HBV tDNA in cancer tissue were higher than cancer-adjacent tissues (t=2.274,P=0.025) and CHB infection group (t=2.367,P=0.011),There was no significance difference in ration of that between CHB infection group and cancer-adjacent group(P = 0.752); (4)Hepatocyte HBV cccDNA were discovered in cancer tissues and cancer adjacent tissues form 4 HCC patients with sera HBV DNA,HBsAg negative and HBcAb positive , the ration of HBVcccDNA/HBV tDNA were higher, maximum reached 0.973; (5) There no significance difference in the load of HBV cccDNA, HBV tDNA among 3 patterms of HBcAg and HBsAg expression in 3 groups respectively (all P > 0.05). (6) Hepatocyte HBV cccDNA and HBV tDNA were not associated with degree of inflammation and of fibrosis in CHB infection group and cancer-adjacent group repectively.(all P > 0.05).Conclusions: (1)When the HBV replication is active, the load of sera HBV DNA was high correspond to hepatocytes HBV cccDNA , But for HCC patients related to HBV, although hepatocyte cccDNA transcripted rc DNA slowly or cessation, even serum HBsAg negatie, HBV DNA undetectable, HBV cccDNA may remain in high level in liver cells.(2) That HBV induce hepatocarcinogenesis may be contributed to HBVcccDNA rather than rcDNA(3)The expression patterns of HBsAg and HBcAg are not sensitive to reflect hepatocyte cccDNA, tDNA dynamic.HBsAg.(4)Hepatocyte HBV cccDNA and tDNA HBV did not led to liver tissue lesions directly.