Preliminary Study On The WWOX Gene In Nasopharyngeal Carcinoma

Objectiveâ‘ To investigate the expression of WWOXmRNA in nasopharyngeal carcinoma and to analyze its relationship with the clinical features and to explore its possible role in NPC.â‘¡To investigate status of WWOX gene promoter methylation in nasopharyngeal carcinoma to analyze its relationship with clinical features, WWOXmRNA expression.â‘¢To investigate status of WWOX gene loss of heterozygosity in nasopharyngeal carcinoma to analyze its relationship with clinical features, WWOXmRNA expression.â‘£To preliminary explore the relationship between expression of WWOXmRNA,promoter methylation and loss of heterozygosity.MethodRT-PCR,methylation specific polymerase chain reaction (MSP),denatured polyacrylamide gel electrophoresis and single strand conformation polymor phism were employed to determine the expression of WWOXmRNA, promoter methylation status and LOH of WWOX gene in nasopharyngeal carcinoma.Resultâ‘ The nasopharyngeal carcinoma cell line CNE1 and CNE2 the expression of WWOX mRNA were missing, the normal nasopharyngeal cell line NP69 was normal.In the detection of 65 cases of NPC tissues, 31 cases of cancer tissue samples WWOXmRNA absence or very low expression, 43 cases collected at the same time the control group, WWOXmRNA were expressed normally. Statistical analysis showed that tumor tissue and non-tumor tissue expression levels between the two WWOXmRNA There were significant differences (P <0.05). Nasopharyngeal carcinoma patients grouped according to the clinical data, found that WWOXmRNA expression in age, gender, histological type, invasion, lymph node metastasis, distant metastasis difference between the various groups was not significant, P> 0.05; clinical stageâ… ~â…¡was significantly higher than that of WWOXmRNA stageâ…¢~â…£, P <0.05â‘¡The nasopharyngeal carcinoma cell line CNE1 and CNE2 the pomoter of WWOX mRNA were methylation, the normal nasopharyngeal cell line NP69 was non-methylated.The positive rate of WWOX promoter methylation in NPC tissues was 56.9%(37/65), in contrast, all of chronic inflammation of nasopharyngeal mucosa tissues were negative,respectively (P<0.05)The positive rate of WWOX promoter methylation in NPC had no relationship with sex,age, pathological classification,clinical stage,attack degree,neck lymph node metastasis and transfer. (P>0.05). WWOX mRNA expression and its promoter methylation was negatively correlated (rs =- 0. 582, P = 0. 000).â‘¢The positive rate of WWOX LOH in NPC tissues and chronic inflammation of nasopharyngeal mucosa tissues was 44.6%(29/65) and 4.7%(2/43),respectively (P<0.05) The positive rate of WWOX LOH in NPC tissues was significantly correlated with clinical stage and neck lymph node metastasis,(P<0.05).No relationship existed between LOH and other clinical datas in NPC tissues.The correlation of WWOX mRNA expression and loss of heterozygosity was not significant (rs =- 0. 196, P = 0. 117).â‘£WWOX mRNA expression and gene methylation, loss of heterozygosity was negatively correlated, and significant correlation (rs =- 0. 546, P = 0.000) Conclusionâ‘ Deletion of WWOX mRNA expression was highly occurred in NPC,the deletion rate of WWOX mRNA expression may be associated with clinical stage. The expression of WWOX may be involved in the occurrence,development of NPCâ‘¡WWOX promoter methylation was highly occurred in NPC,the positive rate of WWOX promoter methylation may be associated with the abnormal expression of WWOX..â‘¢WWOX LOH was highly occurred in NPC, the correlation of WWOX mRNA expression and loss of heterozygosity was not significant.â‘£Deletion of WWOX mRNA expression play an important role in the development in NPC. WWOX promoter methylation may be an important mechanism in silencing of WWOX gene expression in nasopharyngeal carcinoma.