Changes Of Hippocampal Akt/FKHRL1 Signaling Pathway In Rat Following Acute Stress

[Objective]:To investigate the pattern of the Akt/FKHRL1 signaling pathway'change in acute stressed rats; investigating the psychological-stress model of rats.[Materials and Methods]:1.Animals:Adult male Sprague Dowley rats (280±20) g were purchased from Zhejiang University Laboratory Animal Center,housed in group of 5, and were breeded by the workers there.2. Reagents:①First antibody P-Akt (Ser-473),Akt (Ser-473),P-FKHRL1 (Thr-32),FKHRL1(Thr-32),secondary antibody (goat-anti-rabbit antibody);②ECL kit (enhanced chemiluminescent system);③ACTH KIA Kit (Rat mouse).3. Methods:All rats were grouped randomly after 7 days' feeding:control group (C), body-stressed group (S), psychological-stressed group (PS).The S group were stressed by electric shock,The PS group were stressed by witne-ssing other rats being shocked by electricity to produce the fear emotion, Expression of Akt/FKHRL 1 signaling pathway, plasama adrenocortico-tropic hormone (ACTH) and CORT after stress were observed and compared between six groups. Expression of Akt/ FKHRL1 signaling pathway were examined by western-blotting, Plasma ACTH levels were determined by radioimmunoassay.The data were analyzed by SPSS 16.0 for windows. Expression of Akt/FKHRL1 signaling pathway, Plasma ACTH concentrations was assessed by One-Way ANOVA, P<0.05 was considered statistically significant.[Results] electric-shocked rats showed obvious changes in palasma ACTH, which means the body-stress-model was successfully made. There was no significant change of the activity of Akt/FKHRL signaling pathway. Psychological-stressed group showed obvious behavior changes but no significant change in palama ACTH.[Conclusions] 1.Our research reveals that electric foot shock can be a successfal acute stress model; but the Akt/FKHRL1 signaling pathway didn't show significant change which suggested that it needs more research on if the Akt/FKHRL1 signaling pathway acts in the regulation procedure of stress; 2. If by making rats witnessing other rats' suffering can be a succesful psychology-stress model needs to be affirmed.