Research On Several Immune Molecules On Peripheral Blood Mononuclear Cells Which May Play An Important Role In The Aggravation Of Chronic Hepatitis B

Severe hepatitis is a serious clinical syndrome due to necrosis of large numbers of hepatocytes. Currently we still have no specific and effective clinical treatment. Unless given the emergent liver transplantation, most patients will have a poor prognosis. So far, the molecular mechanism of the aggravation of hepatitis-necrosis of large numbers of hepatocytes in a short period-is not clarified. The main cause of the inscrutability of severe hepatitis is lack of sensitive and reliable indicator which can monitor the development of the disease. Therefore, it is extremely important to establish an early warning system for severe hepatitis.In this study, we selected 39 immune molecules including leukocyte differentiation antigen, chemokine receptors, apoptosis-related ligands and receptors, adhesion molecules and Toll-like receptor which may be related to the aggravation of severe hepatitis, hoping to screen several molecules which may probably be indicators to severe hepatitis.We used Real-time polymerase chain reaction to detect the relative expression of 39 immune molecules at mRNA level. Then we found that the expressions of TRAIL, DR5, CD64, CD30, CD27, CD28, CD62L, ICAM-1 and CXCR2 had significant differences between four groups and DR5 was highly associated with the severity of hepatitis. So we further detected DR5 protein expression using Western blot, and found that the expression level of DR5 protein increased along with the aggravation of hepatitis. It is suggested that DR5 could be a potential indicator of great benefit to Physicians to accurately diagnose the stage of disease and to give best individualized treatment for each patient.