Effects Of Tobacco Of Human Osteoblasts Attachment And Proliferation On Titanium Implants

Objiecives:The aim of this work was to investigate the effect of smokingless tobacco extract (ST) on munber, attachment, morphology, and proliferation of human osteoblasts on titanium implants in vitro.Methods:Human osteoblasts on titanium implants were cultured in presence of ST at various concentrations. Observe the morphologic changes of osteoblasts with different concentration of ST under the invert microscope. The proliferation and the attachment of cells were analyzed by MTT method. TThe activity of alkaline phosphatase (ALP) was measured by di-sodium phenyl phosphate.Results:The size of cells became smaller and their shape changed when the concentrations of ST incread. The growth, attachtment and ALP activity were dose-depently inhibited.Conclution:The deleterious effects occurring at bone/titanium implant interface is an important factor leading to the failuret of implantation.