| ObjectiveTo observe the effects of Qingwen Baidu decoction on NF-κB activation in rats lung tissue and the content of IL-1β,IL-13 in blood serum with acute lung injury induced by LPS, so to explore the treatment and the intervention mechanism for acute lung injury.Methods1.144 male rats were randomly divided into six groups, and each group have 24 rats: normal control group, model group, prednisone group, and three Qingwen Baidu decoction groups. Then instill LPS solution (2mg/Kg) into trachea to prepare for ALI model once a day and for two days. After one hours later, use 4 ml drug solution diluted by normal saline to intragastric administration twice a day. Kill 6 rats and collect specimen in each group at 24h, 48h, and kill the others at 72 hours after administration.2. Through examine the total white cell in bronchoaleolar lavage fluid (BALF), wet/dry ratio of lung tissue and the lung index changes, and the pathological changes under light microscope with HE stained rats lung tissue to evaluate the degree of injuried lung and the therapeutic effect of Qingwen Baidu decoction to the ALI.3. Using ELISA method to detect the content of IL-1βand IL-13 in rats serum.4. Using Western Blot method to analyse the expression level of NF-κB in rats lung tissue.5. Using Spssl3.0 statistics software to analyze and process data.Results1. The pathology of rats Lung tissue with LPS induced ALI results the alveolar structure damaged or consolidation, the lung interstitial edema obviously, the alveolar cavity and the lung interstitial were infiltrated by the focal inflammatory cells and red blood cells. And the total white cell in the BAIF, Wet/dry ratio of lung tissue and the lung index were increased significantly. Compared with the normal control group has significant or very significant difference (P<0.05, P<0.01);2. The white cell in the BAIF, the pathological classification of inflammation (except low dose group of 72h) and the lung index were decreased in all doses of Qingwen Baidu decoction treatment groups. And the damaged alveolar, the infiltrated inflammatory cells and erythrocyte exudated were gradully improved. And the wet/dry ratio of large doses of Qingwen Baidu decoction was decreased obviously. Compared with the model group phase has significant or very significant difference (P<0.05, P<0.01);3. The content of IL-1βin rats serum decreased (except low dose group of 24h), and the IL-13 in blood serum rised in all doses of Qingwen Baidu decoction treatment groups. Compared with the model group phase has significant or very significant difference (P<0.05, P<0.01);4. The expression of NF-κB p65 in high and middle dose of Qingwen Baidu decoction treatment groups decreased in 48h, and all does of Qingwen Baidu decoction decreased in 72h. Compared with the model group phase has significant or very significant difference (P<0.05, P<0.01);5. Except the total white cell in the BAIF in 24h and the the expression of NF-κB p65 in the lung tissue higher than prednisone group(P<0.05), Other observational indexes in large does of Qingwen Baidu decoction has no significant difference (P>0.05)Conclusion1. The Qingwen Baidu decoction can reduce the injured degree of ALI rats tissue, and can reduce the inflammatory cells gather, infiltrate and exudate in the lung tissue, so to play a clearer role in lung protection.2. The Qingwen Baidu decoction can accommodate the express of inflammatory cytokine IL-1βand anti-inflammatory cytokine IL-13 to promote the inflammation and anti-inflammatory cytokines have a dynamic balance, reduce the lung inflammation so to have the function of repair and protect the injured lung tissue.3. The Qingwen Baidu decoction can effectively reduced the express of NF-κB in LPS induced ALI rats tissue. By inhibit the activation of NF-κB and the generation of inflammatory cytokines can reduce the inflammatory response in rats lung tissue.4. Using the Qingwen Baidu decoction early can effectively reduced lung tissue injured degree and can reduced the inflammatory response in ALI rats lung tissue induced by LPS.5. Compared with the group of prednisone, applicating the high-dose Qingwen Baidu decoction can achieve similar efficacy with the time prolonged.
|
PDF Full Text Request