| Cardiovascular disease refers to heart and vascular abnormalities consisting mainly of a group of circulatory system diseases, the most serious hazards to human health is one of the diseases, and is one of the leading causes of death in developed countries. Many studies showed that free radicals associated with nervous system diseases. aging, cardiovascular disease, diabetic vascular complications and other disease related.Nifedipine has been widely used in the treatment of to hypertensive patients to reduce the prevalence of strokes, congestive heart failure. myocardial infarction, and sudden death. In the present study this drug was investigated in the antioxidant capacity on rats in vivo and ability of scavenging free radicals in vitro.The aim of the study was to find a kind of medicine which not only can decrease blood pressure, but also can prevent oxidative damage in the body, what would be beneficial to delay the development of hypertension and its complications and improve prognosis.Objectives:This study was to determine whether nifedipine could increase the activity of antioxidant enzyme systems of normal rat in vivo and whether nifedipine could scavenge free radicals against oxidative damage in vitro.Method:We detected the ability of nifedipine to scavenge ABTS- and DPPH and against AAPH and H2O2-induced hemolysis and inhibit the O2- produced and compared with Trolox and Vc in vitro. and healthy Wistar rats were taken small doses of nifedipine by intragastric administration for 28d continuously and the changes of CAT. SOD. GSH-Px and MDA in liver tissue and blood serum of rats were detected.Results:The antioxidant capacity of nifedipine in vitro:(1) Compared with the strong free radical scavenger (Trolox and Vc) find that the IC50 of nifedipine scavenges ABTS- and DPPH is higher. (2) Nifedipine can significantly inhibit AAPH-induced erythrocyte hemolysis. the inhibition time was longer with the concentration higher of the test materials.(3) Vc has a strong inhibition on O2- produce. nifedipine compared with VC that the inhibition is weaker. but is stronger than Trolox.(4) In the same concentration, the inhibition of erythrocyte hemolysis rate of nifedipine on 83.52% is stronger than Vc. The results shown that the inhibition of nifedipine in hemolysis by H2O2-induced is better.The activity of antioxidant of nifedipine on normal rat:(1) Compared with the control group, in the nifedipine group the values are higher at the four test time points. Especially at the 28th day the activity of the CAT activity is obviously higher (P<0.01).(2) Compared with the control group. the activity of SOD in serum in nifedipine group was not significantly different.(3) Compared with the control group. in the nifedipine group serum GSH-Px values were significantly higher (P<0.01) at 7d and 28d. With the delivery time slightly the activity of GSH-Px increased.(4) The content of MDA in Serum of nifedipine group was lower than the control group and showed significant difference(P<0.05).(5) Compared with control group, the activity of SOD. and GSH-Px in liver of nifedipine group rat was significantly increased (P<0.01). and also the content of MDA was decreased (P<0.05). However. CAT activity in liver of nifedipine group rats was no significant difference compared with control group.Conclusion:(1) Nifedipine is a mild free radical scavenger. compared with Trolox and Vc.(2) Nifedipine can significantly inhibit AAPH-induced erythrocyte hemolysis which indicated that it can effectively inhibit free radical-induced lipid peroxidation.(3) The inhibition ability on O2- of Nifedipine is stronger than Trolox but weaker than Vc(4) The inhibition of nifedipine on hemolysis by H:O2-induced is better than VC(5) Nifedipine increased the activity of CAT. SOD and GSH-Px significantly and decreased the content of MDA in the blood serum and liver tissue of rats.
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