Teh Relationship Between LPL Gene Polymorphism And Interaction Of Environmental Risk Factors And CHD

Coronary heart disease (CHD) is the most common cardiovascular disease, with a complex pathogenesis, high morbidity, high mortality, high recurrence rate and high medical costs and other characteristics, serious risk to human health. Epidemiological and clinical studies have shown that CHD is the Leading cause of death of adult in most developed and many developing countries. In Chinese population, the incidence, prevalence and mortality of CHD also showed an increasing trend. As one of serious diseases which threat to the health of our residents, the mechanism of CHD has not been completely clarified, but researchers generally considered it was caused by environmental factors and genetic factors.Lipid metabolic disorder is an important risk factor of CHD. As a key enzyme in triglyceride metabolism and the rate-limiting enzyme, when the synthesis of lipoprotein lipase (LPL) be affected, the cholesterol levels will increase, what may promote the formation and development of atherosclerosis. Therefore, to reseach relationship between LPL gene polymorphism and CHD is significance in exploring the etiology and pathogenesis of CHD.Objective:Through the epidemiological investigation to reveal the risk factors of CHD; To select the SNPs which determine the disease susceptibility and susceptible genotypes of CHD; To clear the relationship between CHD and interaction of LPL gene polymorphisms and the environment.Methods:In this study, case-control study was used. Cases were 201 CHD patients who were treated in the First Hospital of Jilin University between 2009 to 2011. Controls were 190 healthy people who did Physical examination in the First Hospital of Jilin University during the same period. Both cases and controls were Chinese Han, and there was no blood relationship between individuals. The sex, age are matched in case group and control group. After informed consent,5 ml of fasting venous anticoagulant blood was collected from CHD patients and controls, PCR-RFLP method was used to detect the genotypes of the three SNPs. General demographic data, medical history data, lifestyle data, laboratory data and Physical examination information also had been collected. The data was input in Excel database, SPSS13.0 software package was used for statistical analysis.Results:1. Among cases and controls, body mass index was not significantly different; while the difference of waist-hip ratio between cases and controls was significant difference (P<0.05), the WHR of cases was significantly higher than controls.2. Smoking was significant different between two groups (P<0.05), there was more smokers in the case group (52.91%) than in control group (25.78%). While drinking was not significant different in the two groups.3. Hypertension history was significant different between two groups (P<0.05), there was more people have hypertension history in the case group (52.20%) than in control group (24.56%). Blood pressure in the two groups has shown no significant difference, but the history of antihypertensive treatment in the two groups was significantly different, there was more people have antihypertensive treatment history in the case group (44.00%) than in control group (22.12%).4. Diabetes history was significant different between the two groups(P<0.05), the percentage of diabetes history in case group (24.05%) was significantly higher than in control group (14.04%). However, no significant difference was found in blood glucose between two groups.5. The TC and TG in the two groups was significantly different(P<0.05), control group was significantly higher than case group. History of the hypolipidemic was significant different between two groups(P<0.05), there was more people have hypolipidemic history in case group (12.66%) than in control group (0.88%).6. The difference of stroke history in the two groups was not statistically significant.7. The genotype and allele distribution of the three SNPs (rs328, rs3779787 and rs 10099160) on LPL gene in the two groups were not significantly different.8. Blood glucose, blood lipid and blood pressure were not significantly different in different genotype carriers.9. There was interactions between rs3779787 genotype and smoking, rs1900160 genotype and driking.Conclusions:1. The WHR of CHD patients was significantly higher than controls.Central obesity is a risk factor of CHD, and it may play an important role in the occurrence of CHD.2. The percentage of smokers in case group was 52.91%, while 25.78% in control group, and the proportion of smokers in case group was significantly higher than the controls, smoking is a risk factor of CHD.3. The percentage of cases with history of hypertension and history of diabetes were higher than the control group, hypertension history and diabetes history are risk factors of CHD, and play an important role in the development and progression of CHD.4. Rs328, rs3779787 and rs10099160 sites on LPL gene may not the susceptibility locus of CHD in Chinese Han.5. The LPL gene rs328, rs3779787 and rs10099160 polymorphisms would not affect Chinese Han population's blood glucose, blood lipids, blood pressure levels.6. GT/TT genotype on rs3779787 site may reduce the risk of coronary heart disease which induced by smoking; TT genotype on rs1900160 site may reduce the risk of coronary heart disease which induced by driking.