The Research Of Cyclosporin A Combined Matrine On Bladder Tumor Cells T24/ADM Reversal Of Drug Resistance

Bladder cancer is the most common genitourinary system tumors, accounting for 6% of male cancer, the cause is not fully understood, but with the environment, smoking, and genetic factors, which accounted for most of superficial tumors. The current treatment of bladder cancer is still surgery, chemotherapy, radiotherapy and immune-based adjuvant therapy, given after intravesical instillation is an important means of preventing tumor recurrence, however, after chemotherapy, there are still 45% -70% relapse rate . High recurrence rate after the cause, in addition to bladder tumor cells specific to their biological properties, mainly due to multi-drug resistant bladder cancer cells (Multi-drug resistance, MDR) generation.Many studies have shown that CsA can reverse multidrug resistance of tumor cells, but CsA is a potent immunosuppressant, can already immunocompromised cancer patients to further reduce the resistance, leading to severe infection, severe kidney damage and its of clinical applications, now use more combined with other reversal agents, lower drug concentration to reduce its side effects and low cost of Chinese medicine, tissue toxicity, the safety range, wide and strong, the role of target number of advantages, therefore, Tumor reversal agents has become a hot topic in recent years, studies have confirmed a tumor reverse effect of matrine, therefore, this study combined CsA and Chinese medicine MAT reversal T24/ADM resistance.Partâ… Resistant bladder cancer cells T24/ADM resistance detectionObjective: Recovery T24/ADM bladder cancer cells and drug resistance was detectedMethods: Cryopreservation of the tutor group T24 bladder tumor cells in bladder cancer cells T24/ADM resuscitation success Houjiarue adriamycin (ADM) cultured cells were observed under inverted microscope, morphological changes and growth, MTT assay cells tolerance to the drug flow cytometry the expression of P-gp.Results: T24/ADM resistant cells in the form of slightly larger than the T24 cells, nuclear plasma imbalance. After joining ADM, ADM under the same concentration T24/ADM more than the T24 cell survival between groups with statistically significant, P <0.01. T24/ADM cell P-gp expression was significantly increased, T24 expression is not obvious, the difference was significant, P <0.01.Conclusion: The bladder tumor T24/ADM tutor group established resistant cell maintained its resistance.