Experimental Study And Mechanism Of Reversal Of Multidrug Resistance By Salinomycin In Human Bladder Cancer Cells BIU-87/ADM

【Objective】: To explore the effect of Salinomycin and Doxorubicin on the growth ofBladder cancer cell lines BIU-7/ADM in vitro, and the probable mechanism.【Methods】: Human bladder cancer cell line, BIU一87/ADM were interfered withdifferent concentrations of Salinomycin （0.1μM、1μM、5μM、10μM）andDoxorubicin (10μg/ml）in vitro，the cell growth activity was detected withCell Counting Kit-8method。Cell cycle and cell apoptosis changes weremeasured by means of flow cytometry (FCM),The expressional level in cellmembrane of p-gp was examined by immunocytochemistry. Finally, Theresults were analysised by Using SPSS18.0，groups all using Factorialanalysis and One-Way ANOVA。【Results】: Salinomycin and Doxorubicin significantly inhibited the proliferation ona time and dose-dependent manner(P<0.05). when administered in moderateand higher concentration, Salinomycin combined with Doxorubicindemonstrated synergic effect. FCM showed that the cells were blocked at G0/G1phase by salinomycin and The Salinomycin combined with Doxorubicingroup significantly induced apoptosis contrast with that of the other groupP<0.05）;immunocytochemistry showed that the P-gp expression wassuppressed with Salinomycin. 【Conclusion】: Salinomycin and Doxorubicin could inhabit the growth activity ofbladder cancer lines BIU一87/ADM in vitro.Salinomycin can arrest cancercells in G0/G1phase，induce cells apoptosis，surpress the expression of P-gpin cell membrane,Those are the probable mechanism that Salinomycinsensitizes cancer cells to the effects of doxorubicin. therefore, Salinomycinconbined Doxorubicin may provide a kind of new method in the treatment ofMultidrug resistance of bladder cancer．...