The Relationship Between Tissue Factor And Acute Necrotizing Pancreatitis In Rats

ObjectiveTo discuss the sense of tissue factor in the development of acute necrotizing pancreatitis by observing the expression of tissue factor in acute necrotizing pancreatitis before and after treatment by somatostatin.MethodsSixty Wister rats were randomly divided into sham operation group, model group and treatment group. Acute necrotizing pancreatitis was induced by retrograde injection of 5% sodium taurocholate into the common biliopancreatic duct.2,5,8,11h after operation, a subcutaneous injection of somatostatin(1mg/L) was given to the treatment group that received a dose of 15μg/kg. Sham operation group and model group were injected with saline. Rats were sacrificed at 6h and 12h after operation, blood and pancreas specimens were taken to detect Ca concentration and the TF values of serum at different time points. At the same time, observe the pathological changes of pancreatic tissue.ResultsAt 6h and 12h after operation, compared with the sham operation group, the model group showed obvious severity of pancreatic injury, and the expression of TF was significantly higher than the sham operation group (P<0.05), while Ca concentration was significantly lower than the sham group(P<0.05). At 6h and 12h after operation, the model group had almost no change, but expression of TF at 12h was higher than at 6h (P<0.05), Ca concentration was lower at 12h than at 6h (P<0.05). The treatment group showed decreasing severity of pancreatic injury, and TF was significantly lower than the model group (P<0.05), but slightly higher than the sham group (P<0.05); while Ca concentration was significantly higher than the model group(P<0.05), but lower than the sham group (P<0.05). The treatment group showed decreasing severity of pancreatic injury, expression of TF at 12h was lower than at 6h (P<0.05), Ca concentration was higher at 12h than at 6h (P<0.05). Pathology results showed that the sham group had no pathological damage of pancreas, and the severe damage could be observed in the model group, while, with the treatment time prolonged, the extent of damage mitigation gradually reduced in the treatment group. ConclusionsTF can reflect the severity of ANP. To some extent, it can provide a theoretical basis for clinical diagnosis and treatment of ANP.