Effect Of Somatostatin On TLR Pathway During Liver Injuries In Acute Hemorrhagic Necrotizing Pancreatitis In Rats

Objective: To observe the effect of TLR pathway on liver injuries and discuss mechanism of Somatostatin in acute hemorrhagic necrotizing pancreatitis in rats.Methods: There are two parts: First part: eighty SD male rats were randomly divided into two groups: sham-operated (SO) group (n=40), AHNP group (n=40); Second part: eighty SD male rats were randomly divided into two groups: AHNP group (n=40) and Somatostatin-treateded group (n=40). Animals of SO group were flipped ceca; murine model of AHNP was retrograded injection of artificial bile with 5% STC (0.1 ml/100 g) into the bilio pancreatic duct; theropy rats was injected Octreotide. Rats of SO group, AHNP group and Somatostatin- treated group were sacrificed at 3, 6, 12 h. Samples of liver and blood were taken for analysis. The level of Amy ,ALT and TNF-αreflected the rank of inflammation in liver in pancreatis. The expressions of TLR2, 4 mRNA and NF-κB in the liver tissue were measured by RT-PCR and immunohistochemistry. Peritoneal fluid and mortality were measured.Results: TLR2, 4 mRNA could be detected in liver with low values in SO group (0.020±0.005, 0.024±0.004), but they markedly increased at 3 h in AHNP group (0.097±0.020, 0.342±0.057), peaking at 12 h (0.628±0.146, 1.033±0.172; P<0.05), which increased following the time. The expression of NF-κB in liver also increased at 3 h, but it peaked at 6 h. At the same time, the level of Amy,ALT and TNF-αincreased and the injuries of liver were aggravated. By contrast, treatment with Octreotide could effectively inhibit the expression of TLR2, 4 mRNA (3 h: 0.033±0.06, 0.157±0.03; 6 h: 0.120±0.03, 0.286±0.08; 12 h: 0.229±0.07, 0.710±0.20; P<0.05) and NF-κB expression. The levels of Amy , ALT and TNF-αdecreased and the injury of liver got better. In addition, compared with AHNP group, peritoneal fluid and mortality both were less in the Somatostatin-treatment group.Conclusion: These data suggested that TLR pathway took part in liver injuries in AHNP and Somatostatin was good for AHNP, it may inhibit the effect of the elastase and decrease inflammation by TLR pathway.