| Heat shock protein70 (Hsp70) is highly conserved protein induced in cells exposed to adverseenvironmental, have antioxidant, anti-apoptosis, anti-injury and other functions, involved in thebody tolerance. A few studies show that HSP70 gene polymorphism may affect the expression ofHsp70. It is very important to investigate the relationship between HSP70 and body injury.Objective:In this study, we investigate the relationship between HSP70 and lymphocyte apoptosis, therelationship between HSP70 and lung function injury. To study on the feasibility of HSP70 genespolymorphisms as biological marker of lymphocytes injury and the damage of lung functionsusceptibility.Method:190 cock-oven workers were selected as exposure groups and 153 workers unexposed workerswere selected as control groups. We investigated their general information with uniformquestionnaire. Lymphocyte apoptosis in peripheral blood was determined using Flow Cytometry.Lung function indicators were determined using portable spirometer. HSP70-1 G190C,HSP70-2A 1267G, HSP70-hom T2437C genotypes were analyzed by using the polymerase chainreaction-restriction fragment length polymorphism (PCR-RFLP) technique. The haplotypes werecalculated using PHASE 2.0 software. The interaction risk factors between the gene andenvironmental exposure were determined using logistic regression.Result:There were no significantly difference between early apoptosis and total apoptosis rates ofperipheral blood lymphocytes in different B(a)P levels (P>0.05). Linear regression analysisshowed that urine1-OHP levels and the length of service were the main risk factors of thelymphocyte apoptosis, partial regression coefficients were 0.09 and 0.01. The subjects weredivided into low, medium and high exposure group according to the concentration of urinary1-OHP, the upper bound lymphocyte apoptosis rates of 95% subjects in low exposure group(7.9%) was as the limit to determine positive lymphocyte apoptosis subjects. The results showedthat lymphocyte apoptosis positive rate increased with urine 1-OHP levels. Multivariate logisticregression analysis showed that medium exposure group, high exposure group, night work shiftand the length of service were the main risk factors of the lymphocyte apoptosis, OR (95%CI)were 2.079 (0.868, 4.976),4.674 (1.973, 11.074),2.962 (1.576, 5.570) and 1.058 (1.012, 1.106). Gene polymorphisms analysis showed that there were no significantly difference of lymphocytesapoptosis positive rates between different 1-OHP levels among HSP70-1, HSP70-2, HSP70-homgenotypes (P>0.05). Interactions between genes and environment factors showed differenteffects on lymphocytes apoptosis rates increased. Significant positive-interaction was observedbetween HSP70-2 A/G+G/G genotype and high exposure environmental (γ>1, OReg=11.273,OR95% CI=2.244~56.641) which appeared to act in a sub-multiplicative model. There was alsoobserved significant positive-interaction between HSP70-hom T/C+C/C genotype and highexposure environmental (γ>1, OR eg = 3.718, OR95%CI = 1.293 ~ 10.693) which appeared toact in a super-multiplicative model.Lung function indexes in exposed group were lower than in non-exposure group exceptFEV1/FVC%, the difference were significantly (P<0.05). Between different 1-OHP levels, lungfunction indexes in high exposure group were lower than in low exposure group exceptFEV1/FVC%, and have significantly difference of VC% and FVC% (P<0.05).There were no significantly difference of lung function indexes between exposed group andnon-exposure group among HSP70-1, HSP70-2 genotypes (P>0.05). There were significantlydifference of FVC%,MVV% in exposed group among HSP70- hom T/C and T/C+C/C genotypes(P<0.05). There were also significantly difference of VC% in exposed group among HSP70- homT/T and T/C+C/C genotypes (P<0.05).Multivariate logistic regression analysis showed that HSP70-1 G/C, G/C + C/C genotype werethe main risk factors of lung dysfunction, adjust OR (95%CI) were 2.623 (1.037-6.632) and2.666 (1.086-6.548); HSP70-hom T/C genotype was also the main risk factors of lungdysfunction, adjust OR (95%CI) was 2.304(1.036-5.123).Haplotype analysis showed that the CGT haplotype, CGT/CGT haplotype pairs were risk factorsfor lung dysfunction of the exposure groups, OR (95% CI) were 2.592 (1.103-6.091) and12.173(1.485~99.804).Conclusion:1. The increased of peripheral blood lymphocytes apoptosis of coke oven workers may result incoke oven exposure. HSP70-2 A/G+G/G, HSP70-hom T/C+C/C genotype and high exposureenvironmental show an interaction in lymphocyte apoptosis rates increase.2. Coke oven exposure may increase lung function injury. The gene polymorphism of HSP70-1G/C genotype, HSP70-hom T/C genotype had association with lung dysfunction. Coke ovenworkers who have the CGT haplotype and the CGT/CGT haplotype pair may increase thesusceptibility of lung dysfunction. |
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