Experimental Study On Applying Gabapentin To Prevent Incristine-Induced Peripheral Neuropathic Pain

Objective To investigate the efficacy of gabapentin (GBP) in preventing vincristine-induced peripheral neuropathic pain. And the affects on blood-serum inflammatory factors, such as tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6).Methods Eighty female mice were randomly divided into four groups (n =20) namely control group (group NS), neurophathic pain control group (group Pc), low-dose GBP group (group S) and high-dose GBP group (group L). The animals in the four groups were treated with normal saline, vincristine , vincristine plus low-dose GBP and vincristine plus high-dose GBP , respectively. The respond time was measured one day before and one day after the treatments .The heat hyperalgesia was used as indicator, the modification of structures in sciatic nerve was detected by using LM and EM. And we observed the change of TNF-α,IL-6 and NA after the treatment.Results①The body weight of group NS was normal growth. The body weight of group Pc was normal growth early days, and decrease later stage. It was significantly lower than that in group NS. Part of the mice showed symptoms of diarrhea. The body weight of group L was significantly higher than that of group Pc (P<0.05). Four groups of mice had no obvious motor dysfunction performance.②The heat hyperalgesia respond time in group Pc was significantly shorter than the baseline and that in group NS (P<0.05). But compared to the NS group, there is no difference in group S and L. The heat hyperalgesia respond time after treatment in group L was significantly longer than that in group Pc(P<0.05) .③The histological study showed retrogression of sciatic nerve, demyelination and destruction of Schwann cells in group Pc . Compared to group Pc , group L showed minor changes .④The change of inflammatory foctors: Compared with the NS group and previous administration, TNF-αand IL-6 in serum were significantly increased after administration (P<0.05); Compared with the PC group, TNF-αand IL-6 in serum of group L were significantly decreased (P<0.05).Conclusion The animal modal we adept accorded with the basic character of neuropathic pain. Gabapentin is effective in preventing vincristine-induced peripheral neuropathic pain. To a certain extent, gabapentin can reduce the expression of blood-serum inflammatory factors, such as TNF-αand IL-6.