Analysis The Safety And Correlated Factors With Liver Metastasis Mouse Model From Colon And Rectal Cancer

As the population growth and the arrival of aging society, the incidence of cancer continues to rise at global, particularly in developing countries. Colorectal cancer as one of the common digestive tumor , which the number of new cases is the third at male and the second at female, the deaths in the forefront of cancer mortality. More than half of patients with colorectal cancer will happen liver metastases eventually, which caused great concern in the medical profession. Although,combing the surgery with chemotherapy, targeted therapy to the comprehensive treatment of metastatic colorectal cancer has been improved efficacy greatly, the liver metastasis of colorectal cancer remains a medical research and central issue of attention and difficulties . Even the mechanism of liver metastasis of colorectal cancer has not understood fully even. So, safe to estabish the high-fidelity, stable, reliable, reproducible cloning ideal animal model of colorectal cancer liver metastases of colorectal cancer and liver metastasis is a must urgent needed.Objective: To study the establishment and correlated factors of liver metastases animal models from colorectal cancer.Methods: BALB / c mouse were randomly divided into rectum and colon group.After injected CT26 suspension into rectum by the annal or cecum by exploratory laparotomy , perioperative mortality, survival time, weight change, bowel obstruction, bloody ascites, peritoneal seeding, adhesion and other performance factors were observed and compared between colom and rectum group.Results: Perioperative mortality in rectum and colon group were 7.7% and 19.4% respectively (X2=4.78,P=0.029); Average survival time were 25.54±9.24d,33.44±11.58d respectively (t = - 4.49, P = 0.000); Main causes of death in rectum group were obstruction in rectum and colon geoup were 90.3% and 4.3% respectively (X2 =105.17, P = 0.000); Celiac tumor growing rate in colon and rectum group were 64.3 % vs 2.8% (X2= 60.64, P = 0.000); The incidence of hemorrhagic ascites in colon and rectum group were 48.6% and 4.2% respectively (X2=36.32,P=0.000). Intestional adhesion incidence in colon and rectum group were 15.8% and 0% respectively (P=0.000). Liver metastasis rate in rectam and colon group were 29.2% and 51.4% respectively (X2 =7.32,P=0.006). Conclusion:(1) Rectum model is more safe than colon model.(2)Survival time and weight connect with liver metastasis in rectal group,long survival time and less weight difference have a high liver metastasis.(3)The less liver metastases occurred in clon group with bowel adhesion; If bloody ascites found the more liver metastases in colon group.(4)Colon group are higher than rectal group in liver metatasis rate, both of which are verified in situ colorectal cancer liver metastasis model of establishing a viable method.