A Preliminary Study Of Coronary Heart Disease, Diabetic Nephropathy And Breast Cancer Related Gene

Based on Gene chip technology and real-time fluorescence quantitative PCR technology, the pathogenesis of Coronary heart disease (CHD), Diabetic nephropathy (DN) and Breast cancer (BC) were preliminaryly studied at the genetic level.Firstly, we applied microarray chip to screen differentially expressed genes between Hyperlipidemia-CHD (H-CHD) and Non Hyperlipidemia-CHD (NH-CHD),564 differentially expressed genes were screened. After Gene Ontology (GO) analysis and pathway annotation analysis, we found that four significantly enriched GO nodes were involved in biological processes related to lipid metabolism (10.5%), and there were five pathways were involved in lipid metabolism related reactions, six major target genes were found by pathway analysis combined with GO analysis. This research shows CHD have internal reference with lipid metabolism, and target genes could be considered as candidate genes for CHD pre-diagnosis.Secondly, through quantitative detection of gene expression of AGT, AGTR2 and ADRB3 reference to hemodynamics, the experiment investigated the impact of TSF treatment on the expression of susceptibility genes of Diabetic Nephropathy (DN). From the DNIII and DNâ…£patients to the DNIV patients after administration of TSF for 3 months and 6 months, Glomerular filtration rate(GFR) and Microalbuminuria(MALB) levels had improved, all of the three genes expression was continuously close to normal levels. Improvement of gene expression of DNIV patients after 6 months administration of TSF was better than 3 months, suggesting that DN patients is gradually improving metabolic disorders and alleviating the abnormal hemodynamic status by long-term conditioning by TSF.Lastly, the quantitative detection method of Real-time fluorescence quantitative PCR of Breast cancer (BC)-related genes was established. In 17 health persons and 15 breast cancer patients, the expression of VEGF, Bcl-2 and Cox-2 gene were measured. The relevance between gene expression and Breast cancer was illustrated. Tow representative genes which might be potential biomarkers were identified, providing new idea for the research of the mechanism for disease and the development of diagnostic methods.