Protective Effect Of Breviscapine On Kidney In Diabetic Rats Molecular Biology Mechanisms

Objective To study the effct of PH value on developed model of diabetic rats which was induced by streptozotocin (STZ). Methods The rats was Induced by STZ which was dissolve by the 0．9%NS and the acidic or alkalescent citrate. The rate of developed model of the rats and the changes of plasma glucose were monitored. Results 1．The rate of developed model of the rats :the rate of developed model of the rats which was induced by the acidic STZ was 100 % , and the rate of developed model of the rats which was induced with the STZ dissolved by the 0．9%NS was 60 % . The former was higher than the latter. The rate of developed model of the rats which was induced by the alkalescent STZ was 0 % . 2．the plasma glucose of the rats: (1) The rats which was induced by the acidic STZ become the diabetic rats after 48 hours, and their plasma glucose were steady. (2) The rats which was induced with the STZ dissolved by the 0．9%NS become the diabetic rats after 48 hours, but their plasma glucose were decreaseing after a week .And their plasma glucose were decreaseing to order after 4 weeks. (3) The rats which was induced by the alkalescent STZ, but their plasma glucose were not decreaseing all the time. Conclusion 1．PH value was the important factor of developed model of diabetic rats which was induced by streptozotocin (STZ). 2．The rate of developed model in the empty stomach rats which were induced by STZ which were dissolved by the citrate (0．1mol/l, PH4．2-4．5 , consistence2%)are higher. 3．The rats model of short-term high plasma glucose can be induced by the STZ dissolved by the 0．9%NS. Objective To observe the influence of breviscapine on transforming growth factor-β1 in the serum of the SD rat model of diabetes. Then to study the protective effect of breviscapine on diabetic nephropathy. Methods Forty rats were induced into diabetic models by STZ . Then they were randomly divided into model and treatment group, another twenty healthy rats were taken as controls. Rats in the treatment group were given breviscapine. Then the effects of each experimental group were observed by detecting content of the transforming growth factor-β1 in the serum by enzyme linked immuno sorbent assay(ELISA) in 2 weeks or 6 weeks. Results (1) The transforming growth factor-β1 in the serum of the model , the treatment group and the control group discreased in turn. They were 961．91±128．55, 1006．45±131．01, 590．60±276．61 in 2 weeks. Compared with control group, the model and treatment group increased obviously(P<0．05) . They were 1134．93±136．16, 905．46±77．33, 702．15±296．52 in 6 weeks. The difference in them was significative. (2) The creatinine, the BUN and the weight of kidney/the weight of body, of the model and treatment group and the control group discreased in turn. The difference in them was significative in 2 weeks or 6 weeks.Conclusion 1．The transforming growth factor-β1 in diabetic rats were increasing. 2．Breviscapine can suppress the increasing of transforming growth factor-β1 in diabetic rats. 3．Breviscapine can suppress the increasing of the creatinine, the BUN, the weight of kidney/the weight of body in diabetic rats. Breviscapine can protect the kidney of the diabetic rats. Objective The primer concentration of SYBR Green I real-time quantitative polymerase chain reaction detecting expression of P47phox gene mRNA were studied. Methods Made many primer concentration to find the optimum primer concentration of SYBR Green I real-time quantitative polymerase chain reaction detecting expression of P47phox gene. results When the primer concentration was 0．2μmol/L, the result of the PCR was the best. Conclusion revealed the optimum conditions: primer concentration of SYBR Green I real-time quantitative PCR was 0．2μmol/L; The information in this study maybe useful to relative experiment. Objective To investigate the breviscapine's effection on expression of P47phox gene in the kidney of SD rat model of diabetes. Methods sixty rats were randomly separated from three groups: control group , diabetes group, diabetes group treated with breviscapine. Then the expression of P47phox gene in kidney of each experimental SD rat group was detected by SYBR Green I real-time quantitative polymerase chain reaction . The relative data were compared among groups by 2^-4ΔCT method. Results (1) The Ct mean of control group , diabetes group, diabetic group was analyzed, and the difference has significancy (P