The Experimental Study Of Transplant Cells In Different Ways To Treatment Ischemic Brain Injury

Objective To investigate the Acanthopanax induced bone marrow stromal cells (Bone Marrow Stromal Cells, BMSCs) transplantation in rats of different ways of ischemic brain injury the therapeutic effect and mechanisms.Method Adhesion method in vitro application of purified, amplified BMSCs; line of intraluminal middle cerebral artery occlusion model of replication (MCAO). Wistar male rats were randomly divided into modeling is successful will be divided into control group, cell group and the tail vein of transplanted cells in stereotactic transplantation group, and the other, and sham operation group were 4 groups. Nerve function after treatment rats were observed score changes in brain tissue morphological changes in ischemic brain tissue of brain-derived neurotrophic factor (BDNF) expression and the survival of BMSCsResults Cell tail vein graft group and the cell transplantation group stereotactic neurological behavior of rat ischemic cortex pyramidal cell structure than the control group were significantly improved; ischemic cerebral cortex BDNF expression levels have been noticeably enhanced, in which cell transplantation group stereotactic The most obvious improvement; cell transplant group compared with stereotactic cell transplantation group tail vein of the number of transplanted cells survived for more.Conclusion Acanthopanax-induced bone marrow stromal cell transplantation after stereotactic transplantation better able to reduce cerebral ischemic injury caused by the symptoms of neurological impairment.