| Objective:Artificial liver in treatment of acute liver failure has made considerable progress recently. It is a new rapidly developing technology in the field of blood purification. However acute liver failure progresses rapidly, and clinical conditions are very complex. So it is difficult to objectively evaluate the efficacy of artificial liver in treatment of acute liver failure. So we try to construct carbon tetrachloride-induced acute liver failure canine model, study the mechanism of Non-biological artificial liver technology hemoperfusion combined with hemofiltration therapy cure drug-induced acute liver failure, seek new breakthroughs about combined treatment.Methods:Select five female beagle dogs and five male Beagle dogs, the ages of the dogs are 10-12months (13.3±1.18 kg), Randomly divided them into experimental group and control group. Firstly, inject 30% of the acetyl amino phenol (0.5ml/kg)by vein, then inject 40% of carbon tetrachloride(0.5ml/kg) by abdominal subcutaneous.12 hours later, repeat the above medication, but the medication dose is 1/2 of the first administration. The blood perfusion combined with hemofiltration treatment were given to the treatment group after 24 hours, the control group were not given any treatment. Dynamic monitoring the two groups'general conditions, blood(RBC,WBC,PLT),biochemical indicators (ALT,AST,TBIL,CHE,Cr,BUN,TP,ALB,NH3,electrolyte).Results:The treatment can improve the general state of animals, Compared with the control group, The treatment group's biochemical indicators have statistics meanings at the same time points.Conclusions:Construction of carbon tetrachloride-induced acute liver failure canine model is feasible. Although the model is poor repeatable which is also a problem in other models, the model pathophysiological processes similar to clinical ALF, and easy to carry out continuous monitoring. So we provide a new and more ideal experimental animal model for artificial liver research.
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