Screnning Of Gene Fusion In Esophageal Squamous Cell Carcinoma

There is now no substantial evidence for the pathogenesy of esophageal squamous cell carcinoma(ESCC), and even surgery and neoadjuvant chemotherapy can be performed, the overall survival is poor. To solve these problems, we devoted ourselves to the study of gene fusion in ESCC. First, the genome genechips were made to identify translocation breakpoints in 6 cell lines of ESCC, and mapped to around 70kb resolution. Then, we further narrowed the ranges to 600 bp below using quantitive PCR. The genome walking was the critical step, it could help us to catch the unknown break fragment successfully. At last, sequencing was necessary. We identified the t(2;X)(33;26), and PLCL1-FGF13 fusion gene was demonstrated at the same time.Our results suggested that genome walking was the effective way to discover the fusion genes. It breaked with the conventional methods, and was more economical to the experiments in the labs. To date, no fusion genes were discovered in ESCC, PLCL1-FGF13 fusion genes were possible lead to the disorders of FGF signaling, and this chromosomal rearrangement may play an important role during the tumorigenesis. And it would supply a reference to gene therapy in ESCC.