The Expression And Srginficiance Of The Tissue Inhibitor Of C-FOS,MMP-9 And TIMP-1 In Endometriosis

ObjectiveTo investigate the expression of c-fos,matrix metalloproteinase (MMP-9) and tissue inhibitor of metalloproteinase (TIMP-1) and their relationship in ectopic endometriosis tissue and uterine endometrium from women with and without endometriosis, exploring the role of c-fos,MMP-9 and TIMP-1 playing in the the development of endometriosis.MethodsTwenty-seven women with endometriosis were selected as study group. Twenty-seven specimens of ectopic endometrium tissues and eutopic endometrium tissues were collected. Twenty-six specimens of endometrium from reproductive women without endometriosis were obtained as control group. The expressions of c-fos,MMP-9 and TIMP-1 were detected respectively by the immunohistochemical streptavidin-biotin peroxidase (S-P) methodResultsExpression of c-fos,MMP-9 and TIMP-1 were detected in all samples.c-fos was expressed mainly in the nuclei, while mostly cytoplasm expression of MMP-9 and TIMP-1 was observed.The expression of c-fos was significantly higher in ectopic and eutopic endometrium of EMT than in normal endometrium,(Hc=1.9970, P=0.0025); while the expression of c-fos in EMs group between the ectopic and eutopic endometrium tissues had no difference; the expression of c-fos in the proliferative phase was higher than in the secretory phase in both of the EMT group and the control group (p<0.05).3.The expression of MMP-9 in ectopic endometrium of EMT were significantly higher than in eutopic endometrium of EMT and the normal endometrium (p<0.05);Simultaneously MMP-9 expression was significantly higher in eutopic endometrium of EMT than in normal endometrium (p<0.05). 4.The expression of MMP-9 was higher in the proliferative phase than in the secretory phase (p<0.05); while there had no differention of the expression in ectopic endometrium of EMT in different phases of menstrual cycle(p<0.05).5.The expression of TIMP-1 was lower in the ectopic endometrium than in the eutopic endometrium of EMT and the normal endometrium(p<0.05); While TIMP-1 expression was significantly higher in normal endometrium than in eutopic endometrium of EMT (p<0.05).6.The expression of TIMP-1 was higher in the proliferative phase than in the secretory phase (p<0.05); while the menstrual cycle-dependent expression of TIMP-1 was not found in both of the ectopic endometrium and the eutopic endometrium of EMT (P>0.05).7.The expression of c-fos and MMP-9 had a synergy correlation in the three groups of endometrium (rs=0.621, P<0.05).8.There had a mutual restrain relationship between MMP-9 and TIMP-1 in the normal endometrium (rs=-0.580, P<0.05),while in the eutopic and ectopic endometrial tissues of EMT there had no relationship between the two(rs =0.385,P<0.05).ConclusionsThe c-fos,MMP-9,and TIMP-1 were expressed in the eutopic and ecopic endometrium of EMT and the normal endometrium. And c-fos expressed a cyclical endometrium may be related to estrogen and progesterone regulationin the three groups. The higher expression of c-fos and MMP-9 and lower expression of TIMP-1 in ectopic tissue of EMT play an important role in the development of EMT.The interaction of c-fos,MMP-9 and TIMP-1 enhances the ability of the eutopic endometrium to degrade the surrounding matrix,then the endometrium may fall off easily and obtain the strenthener viability to plant in the abdominal cavity after reflux with the menstrual blood,and to infiltrate the surrounding tissue.The c-fos and MMP-9 expression in eutopic and ectopic endometrium of EMT has a positive correlation.They can collaborate with each other to promote the development of EMT.And this variation has nothing to do with the menstrual cycle. While the broken of the dynamic equilibrium between MMP-9 and TIMP-1 also plays an important role in the development EMTThe therapy of endometriosis aimming to modify the biological characters of eutopic endometrium may become the direction of the study. And c-fos,MMP-9 and TIMP-1 may be good marker for etiological treatment of endometriosis.