The Experimental Study Of Nerve Fragments Join NT-3 For Repairing Prolonged Peripheral Nerve Defects

ObjectiveTo observe the nerve fragments join NT-3 induce prolonged peripheral nerve defcts repair role for the clinical encounter cases of peripheral nerve defects to find a way of feasible restoration.MethodWistar rats 40,male, body weight 200g around, randomly divided into 4 groups, each with 10.Anesthetize by ketamine in abdominal, reveal left leg sciatic nerve in sterile conditions,cut apart the nerve in the piriformis margin of about 3mm,which is the sciatic nerve stem about 6mm,retract as their natural. Group A:physiological saline(0.1ml)was added into the biological conduit after neuroma being cut.9/0 non-invasive suture closure of the outer membrane and conduit wall so that making the two ends of the tube from 10 mm.Group B: NT-3 (10ug/ml) was added into the conduit. Group C:The hyperplastic neuroma was cut to the size of 0.5mm3 under the microscope.appropriate fragments were filled conduit,9/0 non-invasive suture outer wall and neural tube after injecting 0.1ml NT-3. Group D:the tibial nerve of proleg was transplanted to the defect area before 9/0 non-invasive suturing the nerve. We suture the wounds and breed one group by one cageResultGeneral observation:Regular observation of postoperative wound and rat gait changes happen and the healing time of ulcers and foot Change; to observe the extent of the left hind limb muscle atrophy, joint stiffness and Exhibition toe flexion reflex situation; the established experimental operation were comple-ted after 4 weeks, exposed nerve after 12 weeks to observe the experimental group, nerve regeneration situation; observation of compatibility between the conduit and surrounding tissue in addition to the situation of nerve anastomosis. the tube wall collapse, or whether twist fracture, whether near or far-side anastomotic enlargement and neuroma formation,tube surface availability for superficial blood formation, the availability of surrounding tissue and adhesions, the improved situation of ulcers and muscle atrophy. By detecting EMG gastrocnemius, triceps surae muscle wet weight, regenerative nerve biopsy study Microstructure and image analysis, the results was applied with SPSS16.0 for one-way ANOVA and t test, P<0.05 for statistical significance, analysis shows: after 12 weeks, The recovery of each group were better than saline group; Nerve fragments+NT-3 group of neurological recovery and autologous nerve graft group better than NT-3 group P<0.05, there was no statistically significance between the two groups, P> 0.05.Conclusion1.Nerve fragments and NT-3 can be used as nerve conduit bridging the filler to promote prolonged peripheral nerve repairing the defect.2.There is a clinically significance to treat prolonged peripheral nerve injury actively.