| Children are special objects of pediatric surgery,and for there own physiological character,the ability of tolerance to operation.is not quite well.Stress reaction reflects the recovery of children post-operation,then leads to the increase the mortality. In the past three decades technique of monitoring pre and post operation,the wildly use of parenteral nutrition which can last for quite a long time,blood transfusion,effective and utility use of antibiotics and so on,all have been greatly advanced.Thanks to these methods,the complication and mortality of the children after operations has been lower than befor.But compared with adult,the rate is still in a high level.As one strong stress,the demage in the operation initiate the stress reaction in the body.The basic reaction is a series change of nervious- endocrinehe.And the most important is the excitement of hypothalamus-pituitary-adrenal cortex system (HPA) The vigious excitement of HPA could be detection by cortisol (Cor) and adreno cortico trophic hormone (ACTH) in blood serum.Nowerdays,some cytokines which were generated by immunocyte cells,like cytokine-6 and so on,could ingress internal secretion-hormonal effect,then aggravate and exacerbate the inflammatory reaction,and impact the body's recovery after the operation.It is the point in pediatric surgery community to to use advanced skill to test the change of these index.Thus,we can't observe the patient's condition and deal with them till make investigate the change of internal environment in stress reaction. In home and abroad,investigation of stress reaction all about animal or human,and the one about both of them can not found.So in my study,I investigate the both,and probe the change of internal environment in stress reaction.ObjectiveThe changes of ACTH,Cor and IL-6 in young rabbit blood serum and children blood serum pre and post operations are all studyed in my research,then analyze these indexes's variation and regularity,and probe investigation of stress reaction. 1 The part of animal experiments1.1 Object of research20 Oryctolagus cuniculus,3-4 month old,no metter they were female or male,weigtht range from 1.1kg～1.4kg.1.2 Reagents and equipmentsRadioactive immune analysis reagents of ACTH and Cor were produced by Kemeidongya biotechnology limited company in Beijing.When worked,as the directions told us to do.The unit of ACTH is pg/ml,and the unit of Cor is ng/ml. Enzyme-linked immunosorbent assays reagent of IL-6 was produced by R&D biotechnology limited company in America.When worked, as the directions told us to do.The unit of IL-6 isμmol/L.Operating-table for animal was offered by laboratory animals centre of Henan Province.1.3 Collect specimenDrew blood in peripheral vein 2ml,then put in into test tube.The time to draw blood were at 24 hours pre-operation,4 hours,24 hours,48 hours,and 72 hours post-operation.The test tube with peripheral vein centrifugated for 10 minites at about 3000 rounds per minute.Then got the serum cryopreservated in fridge at-80°.1.4 Statistical analysisThe value of pre-operation was in normal group,and the value of post-operation was in contrast group.SPSS13.0 Software was used to analyze the date,and results were expressed as(x±s),with the method of independent-sample t test. There was a statistical significance whenα=0.05. 2 The part of human body research2.1 Obj ect of research14 children,3 year-6 year old,who all have operations in abdominal region,no metter they were boys or girls.2.2 Reagents and equipmentsRadioactive immune analysis reagents of ACTH and Cor were produced by Kemeidongya biotechnology limited company in Beijing.When worked,as the directions told us to do.The unit of ACTH is pg/ml,and the unit of Cor is ng/ml. Enzyme-linked immunosorbent assays reagent of IL-6 was produced by R&D biotechnology limited company in America.When worked,as the directions told us to do.The unit of IL-6 is pg/ml.The equipments in the operating room of the Third Affiliated Hospital of Zhengzhou University were used in my research.2.3 Collect specimenDrew blood in peripheral vein 2ml,then put in into test tube.The time to draw blood were at 24 hours pre-operation,4 hours,24 hours,48 hours,and 72 hours post-operation.The test tube with peripheral vein centrifugated for 10 minites at about 3000 rounds per minute.Then got the serum cryopreservated in fridge at -80°.2.4 Statistical analysisThe value of pre-operation was in normal group,and the value of post-operation was in contrast group. SPSS13.0 Software was used to analyze the date,and results were expressed as(x±s),with the method of independent-sample t test. There was a statistical significance when a=0.05.Results(1) In both the part of animal experiments and the part of human body research, the blood serum of ACTH,Cor and IL-6 all rised 4 hours post-operation.(2) Cor and IL-6 in blood serum could decreased to pre-operation levels,but the content of ACTH in blood serum was still more than that of pre-operation's.Conclusions(1) The change of ACTH,Cor and IL-6 in blood serum pre and post operation in animal was suit for children pre and post operation.(2) The change of ACTH,Cor and IL-6 means that the organism was in stress state.(3) The change of Cor and IL-6 in blood serum can reflex the ability of tolerance to operation. |
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