Analysis Of Chromosomal Aneuploid On Embryos From Short-time In Vitro Fertilization-Embryo And Rescue Intracytoplasmic Sperm Injection Crycles

Failed fertilization after IVF occurs in 10-20% of cycles.The reason is different and difficult to determine in each IVF-ET cycle.Sperm defect,disturbances in sperm-oocyte interaction and oocyte abnormality have all been proposed as possible causes of failed fertilization after IVF.It is difficult to forecast the result of failed fertilization. As it report, we will obtain the good pregnancy result if choose the appropriate oocyte to rescue ICSI in time. We advise using short-time IVF and rescue ICSI in order to effectively reduce the rate of cycle cancellation.But whether these two kind of technologies itself will affect chromosomal aneuploid of embryo,it is unknown yet.ObjectivesTo discuss whether short-time IVF and rescue ICSI will affect chromosomal aneuploid of embryo,we use two round FISH to analyze embryo's chromosomal aneuploid.We will appraisal scientific two kind of technology's security and provide the theoritical evidence for supporting in clinical practice.MethodsAbandoned embryos for research were recruited from patients who were performed with IVF/rescue ICSI treatment in Reproductive Medical Center in the First Affiliated Hospital of Zhengzhou University from July 2009 to January 2010. All of the patient have given informed consent for their abandoned embryos. To use aspirate-pull method to obtain blastomere form embryo whose zone lysed by Tween-20/HCl+Methanol/glacial acetic acid. To use Tween-20/HCl+Methanol/ glacial acetic acid methods to fix blastomeres. To compare aneuploid rate and differences of each group after two round FISH with probe LSI13,LSI21,CEPX and CEPY.All data were dealed with SPSS 11.0 statistical package byx2test.α=0.05 is considered as test criterion for statistical analyse. We take P<0.05 as the significance difference standard.Results1. Statistical analysis resultWe have retrospective analysis 4082 ovocytes from 314 IVF-ET cycles,6030 ovocytes from 465 short-time IVF-ET cycles and 749 ovocytes from 82 rescue ICSI cycles. The total fertilization rate and the 2PN fertilization rate of short-time IVF group are not lower than the conventional IVF group.The good embryo rate of short-time IVF group is significance higher than the conventional IVF group.The total fertilization rate of rescue ICSI group is significance higher than other two groups. Its multi-PN fertilization rate is significance higher than conventional IVF group.2. Fix and hybridization outcomesThe total number of collecting abandoned embryo is 152. The fixing rate was 91.9%,while signals rate was 96.0%. The signal responds 143 embryois chromosome.3. FISH outcomes of blastomeresThe chromosomal abnormality rate of short-time IVF group is similar with the conventional IVF cycle. The rescue ICSI group has the same result. The diploidy rate every groups' 2PN embryo is stable, but it also has the aneuploid and gomphosis phenomenon. The 1PN embryo also displays the high chromosome two bodies rate. But the chromosomal aneuploid rate of≥3PN embryo in every group is significance higher than 1PN group and 2PN group. Advanced maternal age, more elder may result in higer abnormality rate. In the≥35 year old group,2PN embryo chromosomal abnormality rate is significance higher than the younger group. But number of oocytes retrieved may have no effect on embryonic chromosomal abnormality.Conclusions1. Short-time IVF does not decrease the fertilization rate, and does not increase the embryo chromosomal abnormality. It is a security effective fertilizing method.2. Rescue ICSI after short-time IVF could be useful to save the failed fertilization. It will effectively reduce cycle cancellation rate, and could not increase the 2PN embryos' chromosomal abnormality risk.3. The 2PN embryos are the best choice to transfer. But they also has the chromosomal abnormality rate. Maybe this is the key factor on lower clinical pregnant rate. The 1PN embryos' chromosome from IVF cycles has a high level diploidy rate. Maybe it declares that the 1PN embryos from IVF cycles would be fertilized normally. Embryos derived from≥3PN are not suitable for transplantation, because of the higher chromosomal abnormality rate.4. Advanced maternal age, especially≥35 year; more elder may result in higer abnormality rate. The number of oocytes retrieved may have no effect on embryonic' chromosomal abnormality.