Effects Of Berberine On Serum Adiponectin In Rat Model And Combination Of Simvastatin With Berberine Reduced Serum Lipid

Objective: To investigate the effects of berberine on serum adiponectin and expression of adiponectin receptormin liver of NAFLD rats.To study the therapeutic effects of combination of simvastatin with berberine on the patients with hyperlipemia.Methods:In animal trial,third-two SD rats were randomized into 2 groups:cont- rol group (n=8,on normal diet) and NAFLD group (n=24,on high-fat diet).The NAFLD model was induced by an oral administ ration of high fat diet.A 8-week berberine and simvastatin intervention was given to established NAFLD rats. After 8 weeks of treatment,the Blood lipid,liver and kidney functionswere detected and compared,and the expression of adiponectin accepter mRNA and protein examined with reverse Real-time fluorescent quantitative PCR and ELISA respectively.In clinical trial,a total of 99 patients with hyperlipidemia in our hospital were divided into 3 groups.Group A: SIMVA,Group B:BBR,Group C: combination of BBR with SIMVA.After 8 weeks of treatment,the Blood lipid,liver and kidney functions in the three groups were detected and compared.Results:In animal trial,The serum adiponectin in the model group were decreased,and were all significantly lower than those in the control group (P<0.05).Compared with the pre-intervention level,the plasma concentration of APN was upgraded (P<0.01),while the expression of adiponectin accepter-1 mRNA has notable statistics significance after berberine and simvastatin treatment for 8 weeks(P>0.05).The expression of adiponectin accepter-2 mRNA has statistics significance Compared with the pre-intervention level(P<0.01).After 8weeks of treatment,in the treatment of the patients with hyperlipemia,combination of BBR with SIMVA was more effective than that of the SIMVA or BBR monotherapy,whose markedly effective cases was 1-fold higher than SIMVA alone.The total effective rate of group C was 100%.Serum TC,TG were significantly different before and after simvastatin treatment(P<0.01).Combination therapy reduced serum TC,TG and LDL-c,the level of them were all signifycantly lower than after simvastatin single-used in the hyperlipemia patients(P<0.01).Compare the three groups,in decreasing TC,TG,LDL-c,group C were more effective than group A or group B,which has notable statistics significance (P<0.01).Conclusion: Intervention with berberine and simvastatin was shown to increase the serum adiponectin and adiponectin accepter-2 mRNA in liver.As compared with monotherapies,the combination showed an improved lipid-lowering effect with reduction of serum lipid.The combination therapy display a new and effective regimen for hyperlipemia.