Expression Of SMAR1 And CyclinD1 And Its Clinical Significance In Human Colorectal Carcinoma

[Objective] To investigate the expression of SMAR1 and CyclinD1 protein and its clinicopathological significance in colorectal carcinoma(CRC). The relationship between the expression of SMAR1 and CyclinD1 protein and the prognosis of CRC was also investigated.[Methods] 1. The expression of SMAR1 and CyclinD1 protein in 128 specimens of CRC and 30 specimens of normal colorectal tissue were detected by S-P immunohistochemistry method. And the relationship between these gene expression level and the clinicopathological parameters was analyzed. Then their influence on the prognosis in CRC were explored.2. A total of 61 paired sample of CRC were recruited from the Chinese population. Western-blot analysis were performed to screen SMAR1 and CyclinD1 expression, the relationship between the protein expression level with clinicopathologic characteristics of CRC was investigated by statistic analysis.[Results] 1.The positive rates of expression of SMAR1 and CyclinD1 protein in specimens of CRC compared to normal colorectal tissue were 44.5%vs76.6%, 61.7%vs13.3%, the difference had statistical significance(p<0.05). The expression of SMAR1 protein was correlated with Dukes stage, infiltration degree, lymphatic metastasis and the degree of cancer differentiation(p<0.05); The expression of SMAR1 protein negatively correlated with that of CyclinD1 protein(r=-0.75,p<0.05).The low expression of SMAR1 and high expression of CyclinD1 protein in CRC had poor prognosis.2. The expression level of SMAR1 protein in CRC detected by Western-blot was lower than that in normal group , and negatively correlated with that of CyclinD1 protein(r=-0.727,p<0.05). Their expression level correlated with the Dukes stage, the state of lymph node metastasis and the histological differentiation. The later the clinical Dukes stage and the lower the pathological differentiation, the more obvious, SMAR1 expression downregulated and CyclinD1 expression upregulated.[Conclusion] The SMAR1 gene expression was down-regulated in most human CRC. It may play a key role in the process of invasion and metastasis in human CRC.