Effect Of Probiotics On Intestinal Bacterial Colonization In Premature Infant

Objective To study the effect of probiotics on intestinal bacterial colonization in premature infants and reducing the intestinal infection and promoting early growth & development.It is to provide a theoretical basis for clinical application of probiotics.Methods 1.Obiects:100 preterm infants without congenital malformations , hyaline membrane disease,severe asphyxia and infection diseases who were admitted to our hospital from 2008.12-2009.12 were recruited and randomly assigned into 2 groups(50 cases as therapy group, 50 cases as control group).2.Research methods:1)Data collection:To record the style of delivery,gender,gestation age,birth weight,Apgar score,amniotic fluid and state of maternal health,to observe whether the skin rash and gastrointestinal adverse reactions occurred during the administration of probiotics and to record whether everybody with detail during hospitalization with diarrhea,sepsis and neonatal necrotizing enterocolitis,ect.2)Sample test: Rectal swab culture was done immediately,5d,12d and before discharging during hospitalization. Blood culture was done when sepsis was suspected. 3)Physical examination: To measure and record the anthropometric measurements of preterm infant including body length, bodyweight and the days return to birth weight. 4)Administration method: Control group was only given general treatment and care. Probiotics was administered to the infants in therapy group.5)Date analysis:The dates were analyzed by SPSS11.5 software Results1.The first three bacteria colonization were klebsiella pneumoniaess,Escherichia coli and Enterococcus faecium in the two groups.2.The pathogenic colonization rates in the control group and treatment group had no difference at admission and 5 day after hospital admission(P>0.05).3.The pathogens colonization rate of the treatment group was significantly lower than that of the control group 12d after hospital admission;The pathogens colonization rate of the treatment group continued to decline, but the control group had no significant change before discharging from the hospital.The pathogens colonization rates in two groups had significant difference at the two time points(P<0.05, P<0.01 ).4.The incidence of diarrhea,,septicemia and feeding intolerance of the two groups had significant difference(P<0.05).There were no NEC occurred. 5.It was significant that the growth and development of premature infant in the treatment group was faster than in the control group(P<0.05).Conclusion1.Probiotics can reduce intestinal pathogens colonization rate of the premature infants ,this effect is more evident with administration time to extend. The types of bacteria colonization were not effected by administration of probiotics.2. Probiotics can reduce the incidence of diarrhea , sepsis and feeding intolerance in premature infants .3.Probiotics can promote early growth and development of premature infant through reducing the incidence of intestinal infection and feeding intolerance possibly.