The Effect Of Promoting Cells Growth By Silk Fibroin Material Loading Basic Fibroblast Growth Factor (bFGF)

In recent years, the unique chemical and mechanical properties of Bombyx mori (B. mori) silk fibroin have made this protein polymer highly attractive for biomedical uses, which have better biocompatibility to provide significant feasible proofs for staring silk fibroin-based tissue healing engineering scaffolds, artifical skin and tissue inducing materials. Growth factor has also been concerned in recent years. Basic fibroblast growth factor (bFGF) have many biological activities that stimulate the proliferation of fibroblasts and capillary endothelial cells, promote angiogenesis and wound healing. This paper takes a systemic research on the relationship between the effect of promoting cells growth and histocompatibility of the regenerated B.mori silk fibroin porous materials loading bFGFThe B.mori silk fibroin films loading bFGF were prepared by casting silk fibroin and bFGF mixture solution on a polyethylene plate and air-dried. The attachment, spreading, proliferation and morphology of L929 cell on the B.mori silk fibroin films and porous materials loading bFGF were observed after cells have been seed for 1d, 3d, 5d, 7d, 9d 11d by microscopy and MTT. The results showed that the B.mori silk fibroin films loading bFGF have better proliferation of cells than without loading bFGF films.B.mori silk fibroin porous materials loading bFGF were prepared by freeze-drying. The MTT value of cells on B.mori silk fibroin porous materials loading bFGF was bigger than that of that of B.mori silk fibroin porous materials without loading bFGF as their has the same ratio of cells at the same cultured time. The results from confocal microscopy observation of GFP indicated that cells proliferation and transfection of Ad-VEGF165 are better on B.mori silk fibroin porous materials loading bFGF.The B.mori silk fibroin porous materials loading bFGF were implanted into the skin defect in sprague-dawley (SD) rat in a 30-mm×30-mm gap defect to evaluate the histocompatibility. The defects were repaired using B.mori silk fibroin porous materials loading bFGF and without loading bFGF. The recovery of the defect dorsum was evaluated postoperatively at the 5th, 11th, 15th, and 28th day using macroscopic, histological of HE and Masson stain. In vivo, at 11 days after implantation, morphological results showed that neo-blood vessel were formed in the boundary of the B.mori silk fibroin porous materials loading bFGF and without loading bFGF. With time increasing, the neo-blood vessel became more and more. The results of HE and Masson stain suggested that B.mori silk fibroin porous materials loading bFGF as well as without loading bFGF materials could guide the formation of functional microvessels and induce the regeneration of dermal tissue. Both of two scaffold material would be biodegradation gradually during the regenerative process.