| Hepatocellular carcinoma (HCC) is the sixth most common cancer and the third most common causeof death from cancer worldwide. Although surgery (surgical resection and liver transplantation) is one of the best treatment, but there are still relatively high recurrence rate. Therefore, it is very important to judge the prognosis after surgery in our clinical practice.Periostin, found in recent years,is a multifunctional and macromolecular glycoprotein. It is secreted mainly by osteoblasts and their precursor cells, also by tumor cells and their adjacent cells. The protein, as a part of extracellular matrix protein composition, is closely related with tumor cell adhesion, metastasis and growth.PN is a macromolecular exocrinosity glycoprotein,and the relative molecular mass is about Mr9×104. The protein is insect cell adhesion molecule-isomers of fiber bundles, was named osteoblast-specific factor-2 (OSF-2), contains a signal peptide coding sequence (N-terminal) and four repeat structures (RDS), each repeat structure has two fasciclin I (a kind of expression in the insect nervous system cell-cell adhesion molecules) homologous to the highly conserved sequence. In the human body, it mainly is secreted by osteoblasts and their precursor cells in the periosteum and the periodontal membrane, can promote the proliferation of osteoblast differentiation and periosteal bone precursor cells into the aggregation and adhesion.There are some common tumors, such as HCC, to detect the expression level of PN tissue abnormalities. But no further related research was reported.The protein in normal liver tissue does not express. Our previous gene chip of HCC with PVTT showing expression of PN is very high.And the report aboat the PN study on HCC was rare at present.ObjectiveTo investigate prognostic values of the intratumoral and peritumoral expression of PN patients aftercurative resection.Patients and Methods1,The same surgical team in our instituteperformed curative resection for HCC on 24 consecutive patients, defined as macroscopically complete removal of the tumor, in July,2009. RT-PCR to check the level of PN in HCC and the adjacent tissues at the genetic level; weston blot method to detect the protein expression.2, Expression of PN were assessed by immunohistochemistry in tissue microarrays containing paired tumor and peritumoral liver tissue from 239 patients who had undergone hepatectomy. (239 cases were confirmed by the histopathology are hepatocellular carcinoma).3,Prognostic value of these and other clinicopathologic factors was evaluated by K-M curves and Cox model to assess gene expression analysis and clinical pathological phenotype and liver cancer prognosis.ResultsExpression of intratumor-PN in HCC than it in peritumoral (p<0.001). Expression of PN in hepatocellular carcinoma is associated with tumor size (p=0.004), tumor differentiation (p=0.045) and TNM staging (p=0.027). The patients with low expression of intratumor-PN has the higher overall survival rate (p<0.01) and higher tumor-free survival rate than the patients with high expression (p<0.001), adjacent differential expression in patients'overall survival (p> 0.05) and free survival (p> 0.05) have no statistically significant difference.Conclusion This study indicates high expression of PN in HCC patients is associated with shorter recurrence and survival time.
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