The Related Clinicopathologic Analysis Of S100A9 And Hsp90β Expression In Colorectal Carcinoma And Adenoma

Objective:To study the expression, clinicopathologic significance and relationship of S100A9 and Hsp90 beta in colorectal carcinoma and adenoma.Methods:The expression of S100A9 and Hsp90 beta was determined in 53 cases of normal tissue samples,53 cases of colorectal carcinoma tissue samples,31 cases of colorectal adenoma tissue samples by SP immunohistochemical method and real time PCR. All data were analyzed by SPSS 17.0 statistic software.Results:1. Immunohistochemistry:The positive rate of expression of S100A9 was 61.3% (19/31) in colorectal carcinoma,29.0%(9/31) in colorectal adenoma, and 22.6%(7/31) in normal tissue. Contrasted to colorectal adenoma and normal tissues, the expression of S100A9 in colorectal carcinoma obviously increased (P<0.05). The expression of S100A9 was positively correlated with histopathobigical grads (P＜0.05). As the degree of differentiation of colorectal adenocarcinoma lowered, the expression of S100A9 gradually increased, but unrelated to age, sexuality, location of tumors, lymphonode metastasis, and Duke's stage. The positive expression rate of Hsp90 beta was 45.2%(14/31) in colorectal carcinoma, 22.6%(7/31) in colorectal adenoma, and 19.4%(6/31) in normal tissue. Contrasted to colorectal adenoma and normal tissues, the expression of Hsp90 beta in colorectal carcinoma obviously increased (P<0.05). The expression of Hsp90βwas positively correlated with histopathobigical grads.As the degree of differentiation of colorectal adenocarcinoma lowered, the expression of Hsp90 beta gradually increased (P<0.05), but unrelated to age, sexuality, location of tumors, lymphonode metastasis, and Duke's stage.2. Real time PCR: The relative quantity of S100A9 mRNA and Hsp90 beta mRNA in colorectal carcinoma increased more than in colorectal adenoma and normal tissues (P<0.05), correlateing with histopathobigical grads (P<0.05).As the degree of differentiation of colorectal adenocarcinoma lowered, the relative quantity of S100A9 and Hsp90 beta gradually increased (P＜0.05).3. The expression of S100A9 was identically correlated with the expression of Hsp90 beta in colorectal carcinoma (P＜0.05), but not in normal tissue and colorectal adenoma.Conclusion:1. The positive rates of expression of S100A9 and Hsp90 beta gradually increased in the sequence from normal tissue, adenoma to adenocarcinoma, which might be involved in carcinogenesis and progression in colorectal carcinoma, suggesting both S100A9 and Hsp90 beta may be important markers of reflecting the state of cell differentiation.2. As the degree of differentiation of colorectal adenocarcinoma lowered, the expression of S100A9 and Hsp90 beta gradually increased (P<0.05), but unrelated to age, sexuality, location of tumors, lymphonode metastasis, and Duke's stage.3. In colorectal carcinoma the expression of S100A9 is strong correlation with Hsp90 beta.Combination with pathological observation, the expression of S100A9 and Hsp90 beta may help us to distinguish the degree of differentiation and bionomics of colorectal adenocarcinoma.