Expression Of CDK6in Colorectal Cancer Tissues And Its Clinical Significance

Objective:The present study was designed to detect the expression of CDK6in colorectal cancer tissues, to analyze the differences between colorectal cancer tissues and normal colorectal tissues, and their relationship of the clinicopathological characteristics of colorectal cancer, and to provide the theory foundations for colorectal cancer prognosis and therapy.Methods:18fresh specimens from colorectal cancer tissues and their corresponding normal colorectal tissues far away from the tumor more than5cm were collected(both confirmed by pathology), and examined the expression of CDK6mRNA by real-time qRT-PCR, analyzed the differences between colorectal cancer tissues and normal colorectal tissues.84paraffin embebed specimens from colorectal cancers and their corresponding normal tissues far away from the tumor more than5cm were collected(both confirmed by pathology). Colorectal cancer tissues were graded to well differentiation, moderately differentiation, and poorly differentiation by pathological diagnosis. Colorectal cancer tissues were divided into Dukes A, B, C, and D stages according to the clinical data. Immunohistochemistry was used to detect the expression of CDK6protein in colorectal carcinoma tissues and normal colorectal tissues, and the differences between them and the relationship of the positive rate and intensity of CDK6expression with clinicopathological characteristics of84cases were analyzed.Results:1. The expression levels of CDK6mRNA in colorectal carcinoma tissues[0.4359(0.0748,1.1358)] were higher than those in normal colorectal tissues[0.062(0.0283,0.2645)](P<0.05).2. The positive rate of CDK6expression in colorectal carcinoma group(88.1%) was higher than that in normal colorectal group(10.4%)(P<0.05).3. The positive rate of CDK6expression in infiltration of the serous membrane group(92.6%) was higher than that in subserosal group(68.8%)(P<0.05), also the group of Dukes C+D(100%) was higher than Dukes A+B(75%)(P<0.05), also the group of lymph metastasis(100%) was higher than no lymph metastasis(80.4%)(P<0.05), and the poorly differentiated group(100%) was higher than the well and moderately differentiated group(83.1%)(P<0.05).4. The expression intensity of CDK6in infiltration of the serous membrane group was stronger than that in subserosal group(P<0.05), also the group of Dukes C+D was stronger than Dukes A+B(P<0.05), also the group of lymph metastasis was stronger than no lymph metastasis (P<0.05), and the poorly differentiated group was stronger than the well and moderately differentiated group (P<0.05). 5. The expression intensity of CDK6had correlation with Dukes stage of colerectal cancer(R=0.416, P<0.05).6. The expression intensity of CDK6had correlation with differentiation degree of colerectal cancer(R=0.324,<0<0.05).Conclusions:1.Both of the expression of CDK6mRNA and protein were high in colorectal carcinoma tissues, indicated that CDK6was involved in the occurrence and development process of colorectal cancer.2. The positive rate and intensity of CDK6expression had correlation with clinicopathological characteristics, such as invasive depth, Dukes stage, lymphatic metastasis, differentiation degree, indicated that CDK6may be taken as one of the indicators in evaluating the prognosis of colorectal cancer.3. The expression intensity of CDK6protein had positive correlation with Dukes stage and differentiation degree, the later Dukes stage and the lower differentiation degree, the stronger expression intensity of CDK6in colorectal cancer.