Chemical Copositions And Content Control Method From Stachys Geobombycis C.Y.Wu

Up to present, domestic and foreign reseach reports for Stachys geobombycis C.Y.Wu are very less, which are mostly for the arranement of material. Their medicinal active ingredients, the formulation of quality standards, and the study of pharmacological aspects only limitied to Chinense scholars. Therefore, the isolated new active ingredients from Stachys geobombycis C.Y.Wu and tie research for pharmacological effects of the various compounds may have good prospects.In this study, we have done the following research:The sparation and structure identification of the chemical composition in Stachys geobombycis C.Y.Wu; The anti-tumor activity in vitro filtering and the determination of apigenin-7-(6"-(E)-p-coumaroyl)-β-D-galactopyranoside, which was isolated from Stachys geobombycis C.Y.Wu.11 compounds were obtained from Stachys geobombycis C.Y.Wu, structures of 8 compounds were identified on the basis of chemical evidences and spectral analysis.8 compounds were determined as:Eicosane acid(1),Oleanolic acid(2),Ursolic acid(3),Apigenin-7-(6"-(E)-p-coumaroyl)-B-D-galactopyranoside(4),Hyperin(5),Baicalein (6),Wogonin(7),Baicalin(8). Compound 1,3,4 were isolated from Stachys geobombycis C.Y.Wu for the frist time.MTT method was used to test the growth inhibition of different concentrations of apigenin-7-(6"-(E)-p-coumaroyl)-B-D-galactopyranoside in the HepG2 and HCT-116 cells in vitro. The compound showed stronger inhibitory action on HepG2 and HCT-116 cells in dose-dependent manner. The experiment initial illustrated the anti-tumor activity of flavonoids in Stachys geobombycis C.Y.Wu, which can provided a theoretical basis of clinical application for the anti-tumor effects in Stachys geobombycis C.Y.Wu.At present, there is na official source of reference standard for apigenin-7-(6"-(E)-p-coumaroyl)-β-D-galactopyranoside. We did the purity test for it by HPLC which was separated from Stachys geobombycis C.Y.Wu, by calculating its purity was 97.63%. HPLC was used for the determination of apigenin-7-(6"-(E)-p-coumaroyl)-B-D-galactopyranoside. Chromatographic experiments were performed on C18 column, the mobile phase was methanol and water (50:50), the detection wavelength was 316 nm, column temperature was 40℃, the flow rate was 1.0 mL-min-1. By measuring the regression equation was Y=25774X-45465, the correlation coefficient r= 1.0000, which indicates that it has a good linear relationship at a concentration of 6.25μg-mL-1-100.00μg-mL-1 range.As new medicines, Stachys geobombycis C.Y.Wu was frist used in Feilaokang Capsule in Nanjing Zhongshan pharmaceutical Co., Ltd. The existing medical standards of it in Jiangsu Province, but no determination of quality chnteol items. Initially based on the experimental results, the product calculated by the dried containing apigenin-7-(6"-(E)-p-coumaroyl)-β-D-galactopyranoside should not be less than 0.60%. This study has made some useful exploration to amend the standard for Stachys geobombycis C.Y.Wu. The results show that it can be used for quality control of Stachys geobombycis C.Y.Wu.