Realationship Between The Expression Of MRNA Of H19 And IGF-Ⅱ Imprinted Gene In Newborn Placenta And Birth Weight

ObjectiveTo study the expression of mRNA of the imprinted gene H19 and IGF-Ⅱin newborn placenta, and to discuss its influence on the birth weight.MethodsThe fresh placental tissues from full-term newborn(without trimester of pregnancy complication and placenta and funic abnormality) with normal, high and low birth weight( 12,10 and 8 examples respectively)were collected. The expression of imprinted gene H19 and IGF-ⅡmRNA in the placenta were estimated by real-time fluorescence quantitative PCR.ResultsThe expression of H19 mRNA in placenta was negative correlation to the birth weight (r=-0.403,P=0.027), The expression of IGF-ⅡmRNA was positive correlated to the birth weight (r=0.444,P=0.014); The H19 mRNA expression level in the high birth weight (0.21±0.31) was significantly lower than that in the low birth weight (1.51±2.04) (P=0.013).But the expression level of IGF-ⅡMrna in the high birth weight (2.67±3.41) was significantly higher than that in the low birth weight (0.39±0.33) (P=0.013)ConclusionThe expression of H19 and IGF-ⅡmRNA was significant difference in the placenta of normal,high and low birth weight.Theese two genes may be related to the birth weight,and there may be some realationship between two genes.