The Influence Of Antiproliferative Factor On C-jun And HB-EGF In Bladder Cancer Cell

ObjectiveAntiproliferative Factor (APF) is a by nine amino acids and galactose linked to the small molecular weight, heat-stable polypeptide of the sialic acid sugar, it contains the Frizzled 8 transmembrane segments, mainly in interstitial the urine of patients with bladder inflammation, with a strong anti-bladder epithelial cell growth, while anti-bladder cancer was found with a role in cell growth, especially for T-24 cells (bladder cancer). AP-1 in tumor formation and development process, by promoting cell proliferation, inhibit differentiation and promote invasion and metastasis of tumor cells play a role in such processes。APF role in normal bladder epithelial cells, its levels of c-jun decreased, resulting in activated protein-1 (Activator Protein-1, AP-1) decline in the overall level of its activity decreased, AP-1 regulation of target genes expression, including cell proliferation, differentiation, inflammation, host response and malignant transformation, while the AP-1 activity reduces the impact of the tumor suppressor gene P53 and cell cycle control gene P21 (P21WAF1/CIP1) gene expression, so that cell growth and proliferation was inhibited, the cells appeared apoptosis or death. HB-EGF was expressed in a variety of tissues and cells, such as vascular endothelial cells, skeletal muscle cells, bladder epithelial cells, keratinocytes and tumor cells, and so on, on a variety of cells and promote proliferation of the role of. HB-EGF in bladder tumor cells has a strong role in promoting their proliferation, while the APF has a strong inhibiting HB-EGF function and the role of secretion and thus inhibit bladder cancer cell growth and proliferation. The influence of APF on bladder cancer cell growth and proliferation by MTT. By Western blot, were found in different concentrations of APF activity and no activity of APF on bladder transitional cell cancer cells in vitro. To explore the role of APF in bladder transitional cells (T-24) cell line c-jun and HB-EGF protein expression and its significance. MethodCultured bladder cancer cell T-24 cell line, there will be a non-active activity of APF and APF to take different concentrations were divided into experimental and control groups, effect on T-24 cells. Enzyme unite immunodetection equipment measure the extinction value of each group in OD 490nm by MTT, and to detect the cell growth rate of bladder tumor. Detected by Western blot,the influence of the different concentration of active and inactive APF on c-jun and HB-EGF in bladder cancer cells to further explore the APF on bladder cancer cell growth and proliferation.ResultThe result which is gotten by MTT measure the extinction value of each hole in OD 490nm by enzyme unite immunodetection equipment. It shows that to follow the increasing of APF density, the extinction value in OD 490nm degrade obviously, and the degrade of entinction value has statistical significance (P＜0.05); But the extinction value of adding incompetence APF has not marked change, and it has not statistical significance compare to experiment control group (P>0.05). According to electrophoretic bands derived from the width of the image and brightness of the semi-quantitative analysis shows that with the increase in the concentration with APF activity, c-jun and HB-EGF expression gradually decreased APF activity of different concentrations of a difference between groups was statistically significant (P<0.05), a non-active APF group and the APF Oμg no statistical significance between groups (P> 0.05).ConclusionAPF may affect the AP-1 in the c-jun expression, c-jun direct role in cell growth arrest bridging the cells, APF role of the cells, the c-jun levels significantly decreased, resulting in the overall level of AP-1 decline in, its activity decreased, then the impact of the tumor suppressor gene P53 and cell cycle regulation of gene expression of P21 protein, which inhibits bladder cancer cell growth and proliferation, but also may affect the expression of HB-EGF and APF activity as there is concentration increased, inhibition of cell growth and proliferation are also gradually. Without APF activity and not affect the expression of c-jun and HB-EGF, but also no inhibitory effect on cell growth. Tip APF for the treatment of bladder cancer patients has important value in the treatment of bladder cancer, a new way.