Evaluation Of Frequently Used Methods To Detect Insulin Sensitivity Of High-fat Diet Rats

ObjectiveTo evaluate comparisons between several frequently used surrogate indexes of insulin sensitivity and the euglycemic-hyperinsulinemic clamp experiments in high-fat diet rats.Method30 male Wistar rats were randomly divided into two groups with a regular (NC group,n=15) or high-fat diet (HF group,n=15) for 10 weeks.The euglycemic-hyperinsulinemic clamp experiments,the insulin tolerance tests as well as the glucose tolerance tests were performed and the correlations of the euglycemic-hyperinsulinemic clamp experiments with different types of IR indexes were conducted.ResultThe glucose infusion rate [GIR (0.90±0.15 vs 4.97±0.68)mg·kg-1·min-1, p<0.01] and KITT(24.41±6.77 vs 48.61±4.71, p<0.01)in HF group decreased significantly. The insulin tolerance tests correlated with the euglycemic-hyperinsulinemic clamp experiments significantly (r=0.911, p<0.01).ConclusionThis study demonstrates that the insulin tolerance tests correlated with the euglycemic-hyperinsulinemic clamp experiments significantly.