The Diaphragmatic Function Of Rats In The Course Of Emphysema

PurposeThe passive smoking was used to duplicate rat emphysema model. To detect the diaphragm fiber types in the whole course of emphysema with enzyme histochemistry. The mTor protein was detected by Western blotting techniques, which in order to explore the mechanism of diaphragmatic dysfunction in the molecular biological level.Experimental materials and methodsOne hundred and twenty healthy seven-week-old male Wistar rats were selected for study, weighting 250-280g, which came from the Experimental Animal Center of China Medical University. They were randomly divided into 3 groups:control group (C), emphysema and smoking group (E) and emphysema and smoking cessation group (N). On the very day of emphysema formation, and after 3 months,6 months and 12 months, we will randomly choose 10 rats for experiment. The rats were put into the home-made smoking capsule (volume 0.25 m3), exposure to 10 Huangguoshu cigarettes,1 hour each day,5 days/week, continued to 75 days. The main experimental methods including lung pathology with HE staining, detection of diaphragm muscle fiber with succinic acid dehydrogenase method and mTOR protein detection with Western Blotting. SPSS13.0 software was used for all data analysis. If P<0.05, statistics is of significance.Results1.In 3 months of pulmonary emphysema, and on the day of emphysema, oxidized fibers in E group and N group were significantly higher than that in C group(P<0.05). In 6 months of emphysema, oxidized fibers of N group were significantly higher than that of C group (P<0.05), the proportion of oxidized fibers in E group compared with the control group, there is no significant difference (P> 0.05). In emphysema of 12 months, E group, N group the proportion of oxidized fibers were higher than the control group (P<0.05).2. The age and the proportion of oxidized fiber correlation analysis showed a positive correlation (P<0.05).3. In 3 months of pulmonary emphysema, and on the day of emphysema, mTOR protein in E group and N group were significantly higher than that in C group(P<0.05). In 6 months of emphysema, mTOR protein of N group were significantly higher than that of C group (P<0.05), mTOR protein in E group compared with the control group, there is no significant difference (P> 0.05). In emphysema of 12 months, mTOR protein of E group and N group were higher than the control group (P<0.05).Conclusions1. The ratio of oxidized fibers in the emphysema rat diaphragm with disease progression first increased and then decreased, indicating compensation and decompensation occurred.2. Increasing with age, the proportion of oxidized fibers gradually increased.3. At early stage of emphysema, signaling protein mTOR protein in emphysema group were higher than that in control group.