Adaptive Changes Of Muscle Fiber In The Rat Diaphragm With Chronic Obstructive Pulmonary Disease

ObjectiveCOPD rat model of the specimen to the diaphragm, the diaphragm and the corresponding changes in the pathogenesis of COPD detection, diaphragmatic muscle fibers, including the distribution of muscle fibers enzymatic activity changes, explore the different stages of COPD diseases associated with the function of the diaphragm, and reveal the rule of functional changes in the diaphragm .MethodsTwenty five healthy seven-week-old male Wistar rats, weighing 180-220 g. were randomly divided into five groups, (1) control group (n=5). (2)COPD group (n=20) . In an enclosed plexiglass cabin COPD rat model was made with fumigation methond. Including four groups : A, COPD one-day group (n=5), After the success of the production model, based on the first day. B COPD one-month group (n=5), one month after the success of the model based on production. C, COPD two-month group (n=5), two months after the success of the production based on the model. D, COPD three-month group(n=5), three months after the success of the production based on the model. Rats were killed, and was taken from the diaphragm, using myosin ATPase determination of ATPase activity, ATP dehydrogenase activity were formed brown-black cobalt sulfide particles and precipitation; Determination of succinate dehydrogenase activity(SDH) ,succinate dehydrogenase activity was part of a blue violet precipitation using succinate dehydrogenase (a symbol of mitochondrial oxidative enzyme) activity, and the use of succinate dehydrogenase stained muscle fibers will be divided into two types of oxidative and the glycolytic fiber , the dark muscle fibers were oxidative fiber, glycolytic fiber stained as light. To observe rat diaphragm fiber types of adaptive changes and enzyme activity changes in different stage of COPD, and compared with healthy controls, and compare the differences between groups with COPD different courses. SPSS13.0 data packet analysis was used for statistical analysis using single-factor analysis of variance, if P <0.05. q test would be used again to compare the differences between each two groups, and did A, B, C, D analysis of the four groups, comparing with the intervention factor in any correlation between the measured factors.Results1. COPD rat model of lung tissue staining success : light microscopy, pathological changes were similar to chronic bronchitis and emphysema of the human.2. ATP dehydrogenase activity in COPD group is on a downward trend compared with the control group.3. Succinate dehydrogenase activity in COPD group compared with the control group, there is a trend of increase.4. Comparing the diaphragm muscle fiber types of changes, the results of statistical analysis :(1) The proportion of oxidative fiber type of the four groups of COPD was significantly higher than that of the control group (P<0.05); the ratio of glycolytic fiber was significantly lower than that of the control group (P<0.05).(2) Among COPD , A, B , C group, there was no significant difference (P > 0.05) ;B C D group, there was no significant difference (P > 0.05).(3) COPD D group has significantly higher ratio of the oxidative fiber than that of A group (P<0.05); and the ratio of glycolytic fiber was significantly lower than that of A group (P< 0.05).(4) COPD severity of the disease associated with the proportion of oxidized fiber analysis : there was a positive correlation (P<0.05).Conclusions1. ATP dehydrogenase is the enzyme of using and decomposing ATP. The results showed ATP dehydrogenase activity in rats with COPD tended lower than that of the control group. The utilization of ATP dropped.2. Succinate dehydrogenase (SDH) is a symbol of mitochondrial oxidative enzyme activity. The results showed COPD succinate dehydrogenase activity is on the upward trend compared with the control group , which showed that the mitochondrial oxidation capacity inseased.3. Then after analysis showing SDH/mATPase of diaphragmatic muscle fibers of COPD rats was higher than that of the control group; These results showed that the mitochondrial oxidative capacity , the ability to produce ATP ,of COPD rats diaphragm for ATP utilization was increased.4. Changes in the diaphragm muscle fiber types of statistical analysis showed that COPD rats diaphragm initial oxidative fibers significantly more than the control group, fiber-type converted from the oxidative to glycolytic fiber. Diaphragm muscle fiber type is transformed into a fatigue type that diaphragmatic resistance-fatigue increased.5. In COPD early stage, with the progress of the illness, the proportion of the diaphragm oxidative fiber inseased, fiber-type converted from the oxidative to glycolytic fiber. The severity of COPD associated with the proportion of oxidative fiber analysis shows : the conversion is positively correlated with the severity of COPD. With the increasing severity of COPD , had the greater degree of conversion.6. COPD early stage diaphragm structure and changes in the composition of the enzyme, COPD respiratory muscle fatigue tolerance will become the basis for histology.