| Objective:To investigate the antibiotic resistance and the distribution ofβ-lactmases in 96 isolates of Acinetobacter spp. from five hospitals in Zhejiang ProvinceMethods:96 non-duplicated clinical isolates of Acinetobacter spp. were collected from five tertiary hospitals of five areas of Zhejiang Province since February,2009. The genomic species were confirmed by PCR detection blaOXA-51 and analysis of 16S-23S rRNA gene intergenic spacer sequences. The susceptibility testing of 13 antimicrobial agents was carried out by Kerby-Bauer method. The minimum inhibition concentrations of polymycin B and Colistin were carried out by agar dilution. PCR and sequencing were used to detect carbapenemase genes, extended-spectrum p-lactamases genes and cephalosporinases genes.Results:96 isolates of Acinetobacter spp. contained A. baumannii (75 isolates), genomic species 3 (6 isolates) and genomic species 13TU (15 isolates). The resistance rates of A. baumannii against antimicrobial agents were much higher than those of genomic species 3 and 13TU. The resistance rates of imipenem and meropenem in A. baumannii were 84%. None of imipenem or meropenem resistant isolate was found in genomic species 3 and 13TU. The resistance rates of carbapenem-resistance A. baumannii were higher than those of carbapenem-susceptible islates. blaOXA-23 was the most dominant carbapenemase gens with the upstream insertion element ISAbal. ISAbal was also located upstream of blaampc in most ceftazidime-resistant islates, while which was absent in all ceftazidme-susceptible islates.Conclusion:The antimicrobial agents resistance of A. baumannii is serious in Zhejiang Province and carbapenem-resistance A. baumannii is widely spread. The coexistence of several resistance genes contributes to the resistance to antimicrobial agents in A. baumannii.
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