Association Of MTNR1B Gene With Type Ⅱ Diabetes In The People Of The Han Nationnality Of Jilin Region

Objective: Type 2 diabetes millitus (T2DM) is a complex disease that is resulted from combined effect of polygenic inheritance and multiple factors,and the report about susceptibility genes for T2DM is different between different race and nation.Recent studies in European populations have found that comman variation in MTNR1B gene are associated with an increased fasting plasma glucose and increased risk of T2DM, We select two SNPs, rs10830963 and rs1387153, which are located in intron of MTNR1B gene and 5'region of MTNR1B gene, respectively,in order to investigate the association between MTNR1B gene and T2DM in molecular level in the Han people of Jilin region.Methods: This study adopted the case-control way and divided the samples into two groups,that was T2DM case and nomal control.We used polymerase chain reaction-fragment length polymorphism (PCR-RFLP) to carry out genotyping,the result of genotyping can be seen through agarose gel electrophoresis of 3%,then we can detect the genotypic frequency. In order to ensure the reliablity of the result,we repeated 20% samples and the result was in 100% accordance with the first time.When we research rs10830963,we design a mismatch in the 3'side of the PCR primer in order to make a site for restriction enzyme pvuâ…¡,meanwhile,We sequenced the different genotypes.The genotypic frequency of the two groups was tested by the law of genetic equilibrium to observe whether they have group representations. Comparation of the allele frequency between these two groups was made with chisquare test of SPSS 15.0,for a significance standard of p<0.05.Results: Both of the two groups are genetic equilibrium populations (p>0.05).The G allele frequency of the rs10830963 between the case and the control have a significant difference(x2=4.872,P=0.027),however, the T allele frequency of the rs1387153 did not show any significant difference (x2=0.761,P=0.383),but it exist a difference in the two groups.Conclusion: (1) G allele of the rs10830963 is a risk factor for type 2 diabetes millitus, while T allele frequency of the rs1387153 did not show any statistical significance,but there is a possibility that T allele might be a risk factor for type 2 diabetes millitus.there is a need to expand the sample if we want to get a exact conclusion.(2)MTNR1B gene is a susceptibility gene for type 2 diabetes millitus ,and there is a need for more research to investigate the molecule mechanism of the gene.Studies on the single nucleotide of MTNR1B show that circadian rhythm play a key role in regulating the glucose and energy metabolism. An ultimate goal of susceptility genes identification is to improve understanding of the pathophysiology of disease, and to use the information to improve diagnosis, prevention and treatment.