Effects Of Arsenic Trioxide On Apoptosis And Interleukin-10 Release Of Peripheral Blood Lymphocyte In Systemic Lupus Erythematosus

Objective : To investigate the apoptosis-induced effect and secretary function of AS2O3 on peripheral blood lymphocyte in Systemic lupus erythematosus (SLE) patients,which can provide theoretical basis for the clinical theatment of Systemic lupus erythematosus.Methods : 1. Peripheral bloodlyphcyte from 15 SLE patients and 15 healthy subjects in normal control group were treated with different concerntration of AS2O3,, the apoptosis rate was calculated and analysized after 12 and 24 hours by flow cytometry(FCM). 2. Cells were stained with acridine orange(AO) and Ethidum bromide(EB) after cultured for 12 hours or 24 hours, the appoptosis pictures were taken by fluorescent microscope. 3. Interleukin-10(IL-10) in supernatant liquid were measured with ELISA. The statistical analysis was executed by SPSS 13.0 software, using the repeated measurement analysis of vatiance (ANOVA) and t-test. the t-test. Statistical significant level was considered as"alpha equal 0.05".Result: 1. AS2O3 can induce appoptosis of peripheral blood lyphcyte of SLE patients and healthy subjects. The effect is gradually increasing along with the increasing of concerntrations of AS2O3 and treated time. The appoptosis rate of both the SLE group and the control group increased accordingly. The appoptosis rate of the two groups with different concerntration of AS2O3 were different. In both group, AS2O3 can evoke significant increase of appoptosis rate(P<0.05). Dexamethasone can induce appoptosis of peripheral blood lymphocyte of both group but with no significant difference (P>0.05). AS2O3 has more significant apoptosis-induced effect on peripheral blood lymphocyte in Systemic lupus erythematosus (SLE) patients than dexamethasone, and statistically analysis has significant difference (P<0.05). 2. The luorescent microscope examination found in cells of apoptosis karyopyknosis,orange stained cellular nucleus. The dead cellular nucleus were also orange stained but did not shrink; Normal cell were only stained green by AO. 3. Compared with controll group, secretion of IL-10 by peripheral blood lymphocyte of SLE group were higher in vitro, which can be supressed by AS2O3 , and was not observed in control group.Conclusion: In this study,we demonstrasted that AS2O3 may induce apoptosis of peripheral blood lymphocyte in Systemic lupus erythematosus (SLE) patients. AS2O3 can down regulate the secretion of IL-10, which may be the mechanism of clinical treatment.