Expression And Significance Of Dendritic Cells And Interleukin-6in Peripheral Blood Of Patients With Systemic Lupus Erythematosus

Objective:In this project, we measured the quantitative change of dendritic cells (DCs) subtypes andthe level of IL-6in peripheral blood of SLE patients, aiming at proposing a theoretical basisfor the pathogenesis, development, and targeting therapy of SLE.Methods1. Groups: Thirty SLE patients (from June2011to December2012) were selected as the SLEgroup, while29healthy people were selected as the control group. The exclusion criteriawere set up strictly.2.10ml venous blood (with heparin1mg:5ml for anticoagulation) was drawn from allpatients in a fasting state. Meanwhile, SLEDAI of all SLE patients were recorded.3. The flow cytometry was used to detect ratio of mDCs and pDCs in peripheral blood of allpatients.4. An ELISA kit was used to quantify the level of IL-6in peripheral blood of all patients.Results:1. The ratio of mDCs in peripheral blood of SLE group (6.244±1.957‰) was significantlylower than that of NC group (14.850±7.491‰)(P<0.05). In the correlation analysis, ourresults showed the level of mDCs of the peripheral blood was related to the SLEDAI scorein SLE patients (r=-0.771) with a P value less than0.05.2. The ratio of pDCs in peripheral blood of SLE group and NC group was1.804±0.518‰and1.809±0.596‰respectively. No significant difference was found between the two groups(P>0.05). In the correlation analysis, no relationship was found between the level of pDCsof the peripheral blood and the SLEDAI score in SLE patients(r=0.275) as a P value more than0.05.3. The level of IL-6in peripheral blood of SLE group (9.636±3.852pg/ml) was significantlyhigher than that of NC group (4.433±1.143pg/ml)(P<0.05). In the correlation analysis, ourresults showed the IL-6level of the peripheral blood was related to the SLEDAI score inSLE patients (r=0.671) with a P value less than0.05.4. In peripheral blood of SLE patients, the level of mDCs was mildly related with that of IL-6(r=-0.369) with a P value less than0.05.5. The sensitivity and specificity of peripheral blood IL-6in diagnosing SLE was86.7%and86.2%respectively at a cut-off value of5.865pg/ml.6. The sensitivity and specificity of peripheral blood mDC in diagnosing SLE was86.7%and79.3%respectively at a cut-off value of8.555‰.Conclusion:1．Peripheral blood mDCs of SLE patients may migrate to local tissue,generate cytokine anddamage tissue of SLE patients.That could induce the occurrence and development of thedisease.2. Peripheral IL-6level of SLE patients not only was associated with the pathogenesis of SLE,but also affect the development of SLE disease.3.There was a negative correlation between peripheral blood mDCs level and peripheral bloodIL-6concentration.