| Objective:To detected the protein expression of repair gene XPF in HCC tissues, peri-cancer tissues and normal liver tissues to analyze the role of their abnormal expressions in HCC tissues and analysis of its relationship with clinicopathological parameters and postoperative recurrence and metastasis of time.Methods:The expression of XPF was detected in 226 cases of HCC,65 cases of peri-cancer tissue and 17 cases of normal liver tissue by Immunohistochemical techniques. The correlation between the expression of XPF and the clinicopathological parameters of HCC were analyzed by Chi-square test. The time to recurrence and metastasis of HCC patients were analyzed by the Kaplan-Meier method and compared by the Log-rank test. Factors affecting prognosis of HCC patients were analyzed by cox proportional regression model for multivariate statistical analysis.Results:(1)The positive expression rate of XPF in HCC tissues was 43.4%, significantly higher than 5.9% in normal liver tissues, the difference was statistically significant (p<0.05). The positive expression rate of XPF in 65 patients with stage I was 52.3%, significantly higher than 20.0%of the 65 cases of peri-cancer tissue, the difference was statistically significant (p<0.05). The positive expression rate of XPF in peri-cancer tissues was higher than that in normal liver tissues, but difference was not statistically significant (p>0.05).(2)The positive expression rate of XPF in this group of patients with a family history of liver cancer was 63.0%, significantly higher than 40.7% of another group without a family history of liver cancer (p<0.05). The positive expression rate of XPF in the patients with portal vein tumor thrombus was 66.7%, significantly higher than 41.3% of that without portal vein tumor thrombus (p<0.05). The positive expression rate of XPF in the patients with HBeAg-positive was 71.4%, significantly higher than 41.5% of that with HBeAg-negative (p<0.05). The positive expression rate of XPF in difference age, gender, preoperative AFP levels, size of tumor, T stage and clinical TNM stage groups and so on, respectively, was not significantly different(p>0.05).(3)One hundred and eighty four patients with stageâ… and stageâ…¡were received radical resection. To postoperative 6M,12M,18M and 24M for the group boundaries, The expression of XPF in these groups were compared. The results showed no statistical difference (p>0.05).(4)Median time to recurrence and metastasis of preoperative ALT>2.5 times the normal group, preoperative AST>2.5 times the normal group, tumor>5cm group were significantly shorter than the corresponding control group (p<0.05). Median time of recurrence and metastasis in this group with XPF-positive is 15.7M, that in another group with XPF-negative is 14.0M, the difference of them was not statistically significant (p>0.05). Time of metastasis and recurrence was no significant statistical difference in this group such as age, gender, ethnic and so on (p>0.05).(5)Multivariate survival analysis by cox proportional regression model showed that the preoperative levels of AST, size of tumor were independent risk prognostic factors and affected prognosis of HCC patients (p<0.05).Conclusion:(1)XPF expression in HCC tissues was singnificantly higher than that in peri-cancer tissues and normal liver tissues.(2)The expression of XPF in HCC was significantly correlated with portal vein tumor thrombus, HBeAg, family history of liver cancer and was not significantly correlated with age, gender, preoperative AFP levels, preoperative AST levels, preoperative ALT levels, ethnic, drinking, smoking, number of tumor, size of tumor, T stage and clinical TNM stage.(3)Time of recurrence and metastasis between XPF-positive group and XPF-negative group was no significant difference. The time of recurrence and metastasis of HCC patients was significantly correlated with preoperative ALT levels, preoperative AST levels and size of tumor.(4)The preoperative levels of AST, size of tumor were independent risk prognostic factors.
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