Polymer Surface Micropatterning And Selectively Absorption Of Protein On Patterned Surface

Nowadays, microcontrol of surface properties and microstructure fabrication within micro-or nanoscaled areas plays an increasingly important role in the development of science and technology. A lot of technologies root in the fabrication of new-style microstructure or miniaturization of existing configuration.Micropatterning, which could be used in surface properties microcontrol and microstructure fabrication, could also be used in the control of surface properties related with intermolecule force and surface topology, such as conglutination and friction. Surface micropatterning of materials shows an expansive prospect in many fields. In microelectron field, surface patterned materials can be used as Magnetic storage media. In biosensor realm, framework of the apparatus could be minified with this technology, the SNR and sensitivity could be rised and the detection limit would be reduced greatly.In biological field, biomolecule could be fixed to the aimed areas with this technology, which is very important for the study of few or single biomolecule's behavior. Therefore, immobility of biomolecules within nano or microareas through surface micropatterning of materials has become a hotspot of research.In this paper, polymer materials were been used as the substrate for micropatterns construction. Self-assembled monolayers was used to modify the polymer surface, vacuum ultraviolet(VUV) lights in a wavelength of 172 nm was used to patterned the surface, and then proteins were absorbed on the patterned surface. The main results of the experiment are as follows:(1) Self-Assembled Monolayers deposited on PET surface. After the irradiation with 172nm VUV light, hydroxyl was discovered on PET surface, which provides a foundation for the formation of amino-terminated self-assmbled monolayers. The surface properties would change after the deposition of self-assembled monolayers. The results tested by water contact angle, Ninhydrin and X-ray photoelectron spectroscopy showed that amino-terminate self-assembled monolayers deposited on the irradiated surface.(2) 172nm VUV light was used to pattern the polymer surface, the patterned samples was tested by scanning electron microscope (SEM) and atomic force microscope (AFM). The results indicated that the patterns as the same as the mesh or Mask used in the irradiation on the surface.(3) Biomolecules was absorbed on the micropatterned surface selectively. PEG molecules could be grafted onto the self-assembled monolayers covered surface, and micropatterns could be found on the surface after the irradiation of 172nm VUV light through the cooper mesh or Mask. The surface took on different properties on different areas, regions covered by PEG molecules could exclude the protein absorption while the other regions couldn't. Results tested by laser scanning confocal microscope showed that fluorescent patterns appeared on the patterned surface. This result proved that proteins were absorbed on the surface selectively, and protein patterns could be formed then.In the experiment, functional micropatterns was fabricated on polymer surface by self-assembled monolayers technology and vacuum ultroviolet lithography, and then protein molecules were absorbed on the patterned surface. The research provide us a new method for the immobility of biomolecules selectively.