| Objective:The aim of this study is to investigate the expression levels of GLI-1,FOXM-1 and PTTG-1 in colorectal cancer tissues and their associations with new lymphangiogenesis and lymphatic metastasis.Methods:(1)The expressions of GLI-1,FOXM-1 and PTTG-1 proteins in 50 cases of colorectal cancer tissues and corresponding adjacent tissues were detected by immunohistochemistry.The relationship between GLI-1,FOXM1,PTTG-1 and clinical stage of colorectal cancer was analyzed.Histological differentiation and lymph node metastasis and other clinicopathological data.By using the LVYE-1 marker,we studied lymphatic vessel densities in 50 colorectal cancer patients and analyzed their association with clinicopathological parameters.In addition,the expressions of GLI-1,FOXM-1 and PTTG-1in lymphatic vessel density(LVD)were investigated.(2)We investigated the mRNA expression levels of GLI-1,FOXM1 and PTTG-1 in 50 CRC patients by Real-time RT-PCR and analyzed the relationship between the mrna expression levels of GLI-1,FOXM1 and PTTG-1 and clinicopathological data such as clinical stage,tissue differentiation and lymph node metastasis.Results:(1)GLI-1 expression was detected in 23 of 50 colorectal cancer tissues and the positive rate was 46%,while only 24%(12/50)in adjacent normal mucosa.The expression of GLI-1 in colorectal cancer tissues was higher than that in normal tissues,and the difference was statistically significant(P<0.05).FOXM-1 was positive in 33 of 50 colorectal cancer tissues.The expression rate of FOXM-1 was 66%(33/50)in colorectal cancer and 8%(4/50)in normal mucosa.The expression of FOXM-1 was significantly higher in colorectal cancer tissues than that in normal tissues(P < 0.05).0.05).The positive expression rate of PTTG-1was 62%(31/50)in colorectal cancer tissues,and 18%(9/50)in normal mucosa tissues.The expression of PTTG-1 in colorectal cancer tissues was higher than that in normal tissues,and the difference was statistically significant(P<0.05).(2)The expressions of GLI-1,FOXM-1 and PTTG-1 proteins were significantly correlated with lymph node metastasis(P<001),but there was no significant difference with age,sex,T stage,Dukes stage,tumor location and depth of tumor invasion.(3)The expression of GLI-1 protein and FOXM-1protein in colon cancer tissues showed a close correlation between them(r=0.970,P<0.05).The expression of GLI-1 protein and PTTG-1 protein in colon cancer tissues showed a close correlation between them(r=0.959,P<0.05).The expression of PTTG-1 protein and FOXM-1 protein in colon cancer tissues showed a close correlation between them(r=0.968,P<0.05).(4)In 50 pairs of matched colorectal cancer tissue specimens,the expression of Gli-1 mrna in cancer tissues and adjacent normal tissues were 0.83±0.183 and 0.59±0.134,respectively.The results showed that the expression level of Gli-1 mrna in cancer tissues was significantly higher than that in adjacent normal tissues.(t=2.40,p<0.05).The expression of FOXM-1 mrna in cancer tissues and adjacent normal tissues were 0.91±0.302 and 0.60±0.141,respectively.The results showed that the expression of FOXM-1 mrna in cancer tissues was significantly higher than that in the corresponding paracancerous tissues(t=1.24.P<0.05).The expression levels of PTTG-1 mrna in cancer tissues and adjacent normal tissues were 1.20±0.165 and 0.84±0.134,respectively.The results showed that the expression level of PTTG-1 mrna in cancer tissues was significantly higher than that in adjacent normal tissues(t=1.72,p<0.05).(5)The expression of Gli-1 mrna in colorectal cancer tissues was significantly correlated with lymph node metastasis and DUKE stage(P<0.05),but not with other clinicopathological features such as age,gender,T stage,tumor location and differentiation degree(P>0.05).The expression of FOXM-1mRNA in colorectal cancer tissues was significantly correlated with lymph node metastasis,DUKE stage and depth of tumor invasion(P<o.05),but not with other clinicopathological features(P>0.05).The expression of PTTG-1 mrna in colorectal cancer tissues was only significantly correlated with lymph node metastasis(P<0.05),but not with other clinicopathological features(P>0.05).(6)The correlation between Gli-1 mrna and FOXM-1 mrna expression was r=0.608,P<0.001,and there was a positive correlation between them.There was a negative correlation between Gli-1 mrna and PTTG-1 mrna expression(r=-0.137).But P>0.05 indicated that there was no statistical significance in the correlation analysis.The correlation between FOXM-1mRNA and PTTG-1 mrna expression was r=0.043,P<0.001;There was a positive correlation between them.(7)LVD in CRC with lymph node metastasis was significantly higher than that in CRC without lymph node metastasis.The lymphatic vessel density in the positive expression group of GLI-1,FOXM-1 and PTTG-1 was significantly higher than that in the negative group(P<P < 0.05).O.001).Conclusion:The high expressions of GLI-1,FOXM-1 and PTTG-1 at gene and protein levels in colorectal cancer tissues may have related pathways affecting the occurrence and progression of tumors,which may be closely related to the formation of lymphangiogenesis and lymph node metastasis.Therefore,GLI-1,FOXM-1 and PTTG-1 may be new targets for biological therapy of colorectal cancer. |
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